Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium
| Autor: | José-Ramón Corcuera-Flores, Raquel Castillo-Oyagüe, María-Ángeles Serrera-Figallo, Rocío Velázquez-Cayón, José-Luis Gutiérrez-Pérez, Gabriel Castillo-Dalí, Daniel Torres-Lagares, Maribel Gonzalez-Martin |
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| Rok vydání: | 2017 |
| Předmět: |
Bone Morphogenetic Protein 2
chemistry.chemical_element 02 engineering and technology Methacrylate Bone morphogenetic protein 2 law.invention 03 medical and health sciences 0302 clinical medicine Acid Etching Dental law Humans Femur General Dentistry UNESCO:CIENCIAS MÉDICAS Cells Cultured Titanium Bone mineral Osteoblasts Research Petri dish 030206 dentistry Adhesion 021001 nanoscience & nanotechnology Machined surface Otorhinolaryngology chemistry Surgery Oral Surgery 0210 nano-technology Biomedical engineering |
| Zdroj: | Medicina Oral, Patología Oral y Cirugía Bucal Velázquez-Cayón R, Castillo-Dalí G, Corcuera-Flores JR, Serrera-Figallo MA, Castillo-Oyagüe R, González-Martín M, Gutierrez-Pérez JL, Torres-Lagares D. Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium. Med Oral Patol Oral Cir Bucal. 2017 Sep 1;22 (5):e651-9. |
| ISSN: | 1698-6946 |
| DOI: | 10.4317/medoral.22071 |
| Popis: | Background: The study of osteoblasts and their osteogenic functions is essential in order to understand them and their applications in implantology. In this sense, this study try to study BMP-2 production and bone matrix deposition, in addition to other biological variables, in osteoblasts cultured on a rough double acid-etched titanium surface (Osseotite®, Biomet 3i, Palm Beach Garden, Florida, USA) in comparison to a smooth titanium surface (machined) and a control Petri dish. Material and Methods: An in vitro prospective study. NHOst human osteoblasts from the femur were cultured on three different surfaces: Control group: 25-mm methacrylate dish (n = 6); Machined group: titanium discs with machined surface (n = 6) and Experimental group: titanium discs with a double acid-etched nitric and hydrofluoric Osseotite® acid surface (n = 6). A quantification of the mitochondrial membrane potential, and studies of apoptosis, mobility and adhesion, bone productivity (BMP-2) and cellular bone synthesis were carried out after culturing the three groups for forty-eight hours. Results: A statistically significant difference was observed in the production of BMP-2 between the experimental group and the other two groups (22.33% ± 11.06 vs. 13.10% ± 5.51 in the machined group and 3.88% ± 3.43 in the control group). Differences in cellular bone synthesis were also observed between the groups (28.34% ± 14.4% in the experimental group vs. 20.03% ± 6.79 in the machined group and 19.34% ± 15.93% in the control group). Conclusions: In comparison with machined surfaces, Osseotite® surfaces favor BMP-2 production and bone synthesis as a result of the osteoblasts in contact with it. Key words:BMP-2, Cytoskeleton, cell culture, bone matrix, apoptosis, cell viability. |
| Databáze: | OpenAIRE |
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