Concentration and turnover of intraperitoneal hyaluronan during inflammation
| Autor: | Greta A. B. Edelstam, Ulla B.G. Laurent, Örjan Lundkvist, Toryard C. Laurent, J. Robert E. Fraser |
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| Rok vydání: | 1992 |
| Předmět: |
Male
medicine.medical_specialty Tritiated water medicine.medical_treatment Immunology Intraperitoneal injection Peritonitis Polysaccharide chemistry.chemical_compound Peritoneum Internal medicine Hyaluronic acid medicine Animals Ascitic Fluid Immunology and Allergy Peritoneal Lavage Hyaluronic Acid chemistry.chemical_classification Lagomorpha biology Metabolism medicine.disease biology.organism_classification medicine.anatomical_structure Endocrinology chemistry Biochemistry Scintillation Counting Female Rabbits |
| Zdroj: | Inflammation. 16:459-469 |
| ISSN: | 1573-2576 0360-3997 |
| DOI: | 10.1007/bf00918972 |
| Popis: | Aseptic peritonitis was induced in rabbits by intraperitoneal injection of irritating agents, mainly starch suspensions. The inflammatory response was followed in the peritoneal lavage fluid by cell counts (average increase about 800-fold the first day) and hyaluronan concentration (average increase about 200-fold on the second and third days). The turnover rate of hyaluronan was studied by injecting tritium-labeled hyaluronan intraperitoneally and by following the appearance of tritiated water in serum. In control animals given trace amounts of hyaluronan, half-lives of 1-14 h were recorded. When the labeled polysaccharide had been mixed with 10 mg/ml of unlabeled hyaluronan, the half-life was approximately one day. Rabbits with ongoing peritonitis exhibited half-lives between 1 and 16 h. It was concluded that there was a large individual variation in uptake kinetics, that the removal process could be receptor mediated, and that the increase in intraperitoneal hyaluronan in peritonitis mainly was due to an increased production of the polysaccharide rather than a decreased rate of removal. |
| Databáze: | OpenAIRE |
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