Requirement for a non-specific glycoprotein cytoplasmic domain sequence to drive efficient budding of vesicular stomatitis virus
| Autor: | Hara P. Ghosh, Matthias J. Schnell, Robert Chernish, John K. Rose, Linda Buonocore, Eli Boritz |
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| Rok vydání: | 1998 |
| Předmět: |
Cytoplasm
Recombinant Fusion Proteins viruses Viral budding Molecular Sequence Data Vesicular stomatitis Indiana virus General Biochemistry Genetics and Molecular Biology Cell Line Viral Proteins VP40 Cytopathogenic Effect Viral Viral Envelope Proteins Cricetinae Animals Humans Amino Acid Sequence Serial Passage Molecular Biology Peptide sequence Sequence Deletion Budding Membrane Glycoproteins General Immunology and Microbiology biology General Neuroscience Cell Membrane Virion Viral nucleocapsid biology.organism_classification Molecular biology Transmembrane protein Cell biology Transmembrane domain Vesicular stomatitis virus CD4 Antigens Mutation Research Article |
| Zdroj: | The EMBO Journal. 17:1289-1296 |
| ISSN: | 1460-2075 |
| Popis: | The cytoplasmic domains of viral glycoproteins are often involved in specific interactions with internal viral components. These interactions can concentrate glycoproteins at virus budding sites and drive efficient virus budding, or can determine virion morphology. To investigate the role of the vesicular stomatitis virus (VSV) glycoprotein (G) cytoplasmic and transmembrane domains in budding, we recovered recombinant VSVs expressing chimeric G proteins with the transmembrane and cytoplasmic domains derived from the human CD4 protein. These unrelated foreign sequences were capable of supporting efficient VSV budding. Further analysis of G protein cytoplasmic domain deletion mutants showed that a cytoplasmic domain of only 1 amino acid did not drive efficient budding, whereas 9 amino acids did. Additional studies in agreement with the CD4-chimera experiments indicated the requirement for a short cytoplasmic domain on VSV G without the requirement for a specific sequence in that domain. We propose a model for VSV budding in which a relatively non-specific interaction of a cytoplasmic domain with a pocket or groove in the viral nucleocapsid or matrix proteins generates a glycoprotein array that promotes viral budding. |
| Databáze: | OpenAIRE |
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