N-acetylcysteine attenuates kidney injury in rats subjected to renal ischaemia-reperfusion
| Autor: | Nicoletta Nitescu, Sven-Erik Ricksten, Niels Marcussen, Ulf Nilsson, Gregor Guron, Samar Basu, Börje Haraldsson |
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| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Urinary system medicine.medical_treatment Renal function urologic and male genital diseases medicine.disease_cause Statistics Nonparametric Renal Circulation Excretion Acetylcysteine Rats Sprague-Dawley Reference Values Internal medicine medicine Animals Transplantation Kidney Analysis of Variance business.industry Biopsy Needle Acute Kidney Injury medicine.disease Glutathione Immunohistochemistry Rats Disease Models Animal Oxidative Stress medicine.anatomical_structure Endocrinology Nephrology Creatinine Reperfusion Injury Hemodialysis business Oxidative stress Kidney disease medicine.drug Glomerular Filtration Rate |
| Zdroj: | Nitescu, N, Ricksten, S-E, Marcussen, N, Haraldsson, B, Nilsson, U, Basu, S & Guron, G 2006, ' N-acetylcysteine attenuates kidney injury in rats subjected to renal ischaemia-reperfusion ', Nephrology, Dialysis, Transplantation, vol. 21, no. 5, pp. 1240-1247 . |
| ISSN: | 0931-0509 |
| Popis: | Background. The aim of the present study is to examine the effects of N-acetylcysteine (NAC), a thiol-containing anti-oxidant, on renal function and morphology, and biomarkers of oxidative stress, in rats subjected to renal ischaemia-reperfusion (IR). Methods. Sprague–Dawley rats underwent unilateral nephrectomy and either contralateral renal IR (40 min of renal arterial clamping), or sham manipulation. Treatment groups were: (1) IR-Saline, (2) IR-NAC, (3) Sham-Saline and (4) Sham-NAC. The N-acetylcysteine was administered in a dose of 200 mg/kg intraperitoneally at 24, 12 and 2 h before, and 24, 48 and 72 h after, renal IR. Plasma creatinine was measured on days 1, 3 and 7 after IR, and kidney histology was assessed on day 7. In separate groups of animals we measured renal levels of the anti-oxidant glutathione, markers of systemic oxidative stress (plasma ascorbyl radical, urinary 8-iso-prostaglandin F2a), and glomerular filtration rate (GFR) by 51 Cr-EDTA clearance, on day 1 after renal IR. Results. Treatment with NAC ameliorated the decline in GFR and reduced hyperkalaemia on day 1 (P |
| Databáze: | OpenAIRE |
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