Silk Sericin-Polylactide Protein–Polymer Conjugates as Biodegradable Amphiphilic Materials and Their Application in Drug Release Systems
| Autor: | Kanittha Boonpavanitchakul, Livia K. Bast, Rathanawan Magaraphan, Nico Bruns |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Models
Molecular Polyesters Biomedical Engineering Pharmaceutical Science Bioengineering macromolecular substances 02 engineering and technology 010402 general chemistry 01 natural sciences Micelle Sericin RS Drug Delivery Systems stomatognathic system Amphiphile Animals Humans QD Sericins Pharmacology Antibiotics Antineoplastic Chemistry Organic Chemistry technology industry and agriculture Hep G2 Cells Hydrogen-Ion Concentration respiratory system Bombyx equipment and supplies 021001 nanoscience & nanotechnology Combinatorial chemistry 0104 chemical sciences Drug Liberation SILK Doxorubicin Delayed-Action Preparations Drug delivery 0210 nano-technology Drug carrier Linker Biotechnology Conjugate |
| Zdroj: | Bioconjugate Chemistry |
| ISSN: | 1520-4812 1043-1802 |
| Popis: | Silk sericin (SS) is a byproduct of silk production. In order to transform it into value-added products, sericin can be used as a biodegradable and pH-responsive building block in drug delivery materials. To this end, amphiphilic substances were synthesized via the conjugation of hydrophobic polylactide (PLA) to the hydrophilic sericin using a bis-aryl hydrazone linker. PLA was esterified with a terephthalaldehydic acid to obtain aromatic aldehyde terminated PLA (PLA-CHO). In addition, lysine groups of SS were modified with the linker succinimidyl-6-hydrazino-nicotinamide (S-HyNic). Then, both macromolecules were mixed to form the amphipilic protein-polymer conjugate in buffer-DMF solution. The formation of bis-aryl hydrazone linkages was confirmed and quantified by UV-vis spectroscopy. SS-PLA conjugates self-assembled in water into spherical multicompartment micelles with a diameter of around 100 nm. Doxorubicin (DOX) was selected as a model drug for studying the pH-dependent drug release from SS-PLA nanoparticles. The release rate of the encapsulated drug was slower than that of the free drug and dependent on pH, faster at pH 5.0, and it resulted in a larger cumulative amount of drug released than at physiological pH of 7.4. The SS-PLA conjugate of high PLA branches showed smaller particle size and lower loading capacity than the one with low PLA branches. Both SS-PLA conjugates had negligible cytotoxicity, whereas after loading with DOX, the SS-PLA micelles were highly toxic for the human liver carcinoma immortalized cell line HepG2. Therefore, the SS-based biodegradable amphiphilic material showed great potential as a drug carrier for cancer therapy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|