Bioconversion of ethyl (R)-4-cyano-3-hydroxybutyate into (R)-ethyl-3-hydroxyglutarate via an indirect pathway by Rhodococcus boritolerans
| Autor: | Ming-Jia Yang, Zhong-Yi Yang, Wensheng Xiang, Xiangjing Wang, Ji Zhang, Jing An |
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| Rok vydání: | 2011 |
| Předmět: |
biology
3-Hydroxybutyric Acid Bioconversion Chemistry Stereochemistry Temperature Substrate (chemistry) Bioengineering General Medicine Hydrogen-Ion Concentration Glutarates biology.organism_classification Applied Microbiology and Biotechnology Culture Media Biotransformation Yield (chemistry) Side chain Rhodococcus Metabolic Networks and Pathways Biotechnology |
| Zdroj: | Biotechnology letters. 34(5) |
| ISSN: | 1573-6776 |
| Popis: | (R)-Ethyl-3-hydroxyglutarate, (R)-3, is an intermediate in the synthesis of the statin side chain. Here, a new two-step, indirect biotransformation pathway involving the formation of ethyl (R)-4-carbamoyl-3-hydroxybutanoate, (R)-2, as an intermediate for (R)-3 production was developed using Rhodococcus boritolerans with ethyl (R)-4-cyano-3-hydroxybutyate, (R)-1, as substrate. Maximum conversion was with 10 g (R)-1/l, 7 g cells/l (dry wt), pH 7.5 and 25°C. A yield of 98 ± 0.5% (w/w) was attained within 8 h. |
| Databáze: | OpenAIRE |
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