Effect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival
| Autor: | Fatemeh Haj Manoochehri, Taghi Naserpour Farivar, Dariush Hamedi Asl, Amir Peymani, Babak Rahmani |
|---|---|
| Jazyk: | perština |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Iron lcsh:Medicine Pharmacology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation Medicine FTH1 Sodium bicarbonate biology Tea Helicobacter pylori business.industry Cell growth lcsh:R Metabolism medicine.disease biology.organism_classification 030104 developmental biology Iron-deficiency anemia chemistry Cell culture 030220 oncology & carcinogenesis business |
| Zdroj: | The Journal of Qazvin University of Medical Sciences, Vol 22, Iss 5, Pp 36-43 (2018) |
| ISSN: | 2228-7213 1561-3666 |
| Popis: | Background: Iron deficiency anemia is the most common nutritional disorder in the world. Diet and Helicobacter pylori infection are among the main causes of this disorder. Objective: In this study, the effect of black tea extract and sodium bicarbonate with Helicobacter pylori on the genes involved in iron absorption and storage, as well as cell proliferation, were studied. Methods: Simultaneous cultivation of MDCK and Helicobacter pylori cell lines was performed at concentrations of 10, 20, 40 and 80 μg/ml of tea extract and 30, 40, 60 and 100 mM sodium bicarbonate at 24 and 48 hours. The effect of treatment on cell survival was investigated by trypan blue staining and expression of MYC, TFRC, FTH1, IRP2, IRP1, and NDRG1 genes by real-time PCR and analyzed by ANOVA and independent T-test. Findings: There was no significant change in the expression of the genes involved in iron metabolism under the influence of tea, sodium bicarbonate and Helicobacter pylori treatment in MDCK cell line. Upregulation MYC gene expression was observed in the presence of Helicobacter pylori after 24 hours treatment with tea extract, and sodium bicarbonate, and in the absence of Helicobacter pylori upregulation with tea extract after 48 hours (P |
| Databáze: | OpenAIRE |
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