Suspension Culture of Undifferentiated Human Embryonic and Induced Pluripotent Stem Cells
| Autor: | Idit Blais, Michal Amit, Yael Miropolsky, Joseph Itskovitz-Eldor, Meital Peri, Michel Revel, Victoria Margulets, Ilana Laevsky, Guy Slutsky, Judith Chebath, Kohava Shariki |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
KOSR
Cancer Research Induced stem cells Interleukin-6 Recombinant Fusion Proteins Rex1 Blotting Western Induced Pluripotent Stem Cells Apoptosis Cell Differentiation Cell Biology Embryoid body Biology Flow Cytometry Polymerase Chain Reaction Embryonic stem cell Molecular biology Cell biology Karyotyping embryonic structures Humans Stem cell Induced pluripotent stem cell Embryonic Stem Cells Adult stem cell |
| Zdroj: | Stem Cell Reviews and Reports. 6:248-259 |
| ISSN: | 1558-6804 1550-8943 |
| Popis: | Alongside their contribution to research, human embryonic stem cells (hESC) may also prove valuable for cell-based therapies. Traditionally, these cells have been grown in adhesion culture either with or without feeder cells, allowing for their continuous growth as undifferentiated cells. However, to be applicable in therapy and industry they must be produced in a scalable and controlled process. Here we present for the first time a suspension culture system for undifferentiated hESC and induced pluripotent stem cells (iPSC), based on medium supplemented with the IL6RIL6 chimera (interleukin-6 receptor fused to interleukin-6), and basic fibroblast growth factor. Four hESC lines cultured in this system maintained all ESC features after 20 passages, including stable karyotype and pluripotency. Similar results were obtained when hESC were replaced with iPSC from two different cell lines. We demonstrate that the IL6RIL6 chimera supports the self-renewal and expansion of undifferentiated hESC and iPSC in suspension, and thus present another efficient system for large-scale propagation of undifferentiated pluripotent cells for clinical and translational applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|