Sequential Transcriptome Changes in the Penumbra after Ischemic Stroke

Autor:	Dong-Hee Choi, Ji-Hye Kim, Ji Hee Yun, Hahn Young Kim, In-Ae Choi, Jongmin Lee
Rok vydání:	2019
Předmět:	Male Time Factors RHOA genetic structures acute stroke MAPK8 Population Biology Article Catalysis Brain Ischemia Inorganic Chemistry Transcriptome chronic stroke Animals ischemic penumbra cardiovascular diseases Protein Interaction Maps Rats Wistar Physical and Theoretical Chemistry KEGG Receptor education Molecular Biology GRIA1 Spectroscopy education.field_of_study gene profile alterations Penumbra Organic Chemistry Brain Thrombosis General Medicine stroke Computer Science Applications Cell biology Gene Ontology biology.protein sense organs
Zdroj:	International Journal of Molecular Sciences Volume 20 Issue 24
ISSN:	1422-0067
Popis:	To investigate the changes in the expression of specific genes that occur during the acute-to-chronic post-stroke phase, we identified differentially expressed genes (DEGs) between naive cortical tissues and peri-infarct tissues at 1, 4, and 8 weeks after photothrombotic stroke. The profiles of DEGs were subjected to the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and gene ontology analyses, followed by string analysis of the protein&ndash protein interactions (PPI) of the products of these genes. We found 3771, 536, and 533 DEGs at 1, 4, and 8 weeks after stroke, respectively. A marked decrease in biological&ndash process categories, such as brain development and memory, and a decrease in neurotransmitter synaptic and signaling pathways were observed 1 week after stroke. The PPI analysis showed the downregulation of Dlg4, Bdnf, Gria1, Rhoa, Mapk8, and glutamatergic receptors. An increase in biological&ndash process categories, including cell population proliferation, cell adhesion, and inflammatory responses, was detected at 4 and 8 weeks post-stroke. The KEGG pathways of complement and coagulation cascades, phagosomes, antigen processing, and antigen presentation were also altered. CD44, C1, Fcgr2b, Spp1, and Cd74 occupied a prominent position in network analyses. These time-dependent changes in gene profiles reveal the unique pathophysiological characteristics of stroke and suggest new therapeutic targets for this disease.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b73a6fc3ce817ef53ad6178d7e7afbe5 https://doi.org/10.3390/ijms20246349 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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