AC electrokinetic immobilization of organic dye molecules
| Autor: | Eva-Maria Laux, Christian Wenger, Frank F. Bier, Ralph Hölzel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Molecular dielectrophoresis
Materials science AC electrophoresis Organic dyes Dielectrophoresis Interdigitated electrodes Biochemistry Fluorescence Analytical Chemistry Electrokinetic phenomena Electrophoresis Chemical physics Electric field AC electrokinetics Electrode Organic dye Molecule Research Paper |
| Zdroj: | Analytical and Bioanalytical Chemistry |
| ISSN: | 1618-2650 1618-2642 |
| Popis: | The application of inhomogeneous AC electric fields for molecular immobilization is a very fast and simple method that does not require any adaptions to the molecule’s functional groups or charges. Here, the method is applied to a completely new category of molecules: small organic fluorescence dyes, whose dimensions amount to only 1 nm or even less. The presented setup and the electric field parameters used allow immobilization of dye molecules on the whole electrode surface as opposed to pure dielectrophoretic applications, where molecules are attracted only to regions of high electric field gradients, i.e., to the electrode tips and edges. In addition to dielectrophoresis and AC electrokinetic flow, molecular scale interactions and electrophoresis at short time scales are discussed as further mechanisms leading to migration and immobilization of the molecules. |
| Databáze: | OpenAIRE |
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