Analysis of 2000 consecutive UK tonsillectomy specimens for disease-related prion protein
Autor: | Jonathan D. F. Wadsworth, Carl J Jackson, Jacqueline M. Linehan, John Collinge, Sebastian Brandner, Lorraine C Smith, Adam Frosh |
---|---|
Rok vydání: | 2004 |
Předmět: |
PrPSc Proteins
Prions animal diseases medicine.medical_treatment Bovine spongiform encephalopathy Immunoblotting Palatine Tonsil Disease Creutzfeldt-Jakob Syndrome Prion Diseases Prion infection Cricetinae mental disorders medicine Prevalence Animals Humans Prion protein Child Subclinical infection Tonsillectomy Brain Chemistry business.industry Small sample General Medicine medicine.disease Virology Immunohistochemistry United Kingdom nervous system diseases business |
Zdroj: | Lancet (London, England). 364(9441) |
ISSN: | 1474-547X |
Popis: | Variant Creutzfeldt-Jakob disease (CJD) is thought to be caused by dietary or other exposure to bovine spongiform encephalopathy (BSE) prions. The prevalence of preclinical or subclinical prion infection in the UK is currently unknown. Since clinical variant CJD is uniformly associated with tonsillar prion infection, we screened 2000 anonymous surgical tonsillectomy specimens for disease-associated prion protein. Analysis by both high sensitivity immunoblotting and immunohistochemistry detected no positive cases. However, this negative result cannot provide reassurance that relevant community infection is unlikely because of the fairly small sample size, demographic and age-related factors, and unknown test sensitivity during the prolonged incubation period. Nevertheless, our findings establish a protocol for prevalence screening on a national scale. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |