Structure of the mouse dipeptidyl peptidase IV (CD26) gene
| Autor: | Marie-Genevieve Mattei, Didier Marguet, Anne-Marie Bernard, Michel Pierres |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Sequence analysis
Dipeptidyl Peptidase 4 Molecular Sequence Data Restriction Mapping Locus (genetics) Saccharomyces cerevisiae Biochemistry Dipeptidyl peptidase Mice Exon Animals Amino Acid Sequence Promoter Regions Genetic Chromosomes Artificial Yeast Gene Genomic organization Base Sequence Chemistry Alternative splicing Intron Chromosome Mapping Exons Bacteriophage lambda Molecular biology Introns Alternative Splicing Poly A Sequence Analysis |
| Zdroj: | Biochemistry. 33:15204-15214 |
| ISSN: | 1520-4995 0006-2960 |
| DOI: | 10.1021/bi00254a032 |
| Popis: | Dipeptidyl peptidase IV (DPP IV, EC 3.4.14.5) is an ectopeptidase whose expression is modulated during thymocyte differentiation and T cell activation. We describe here the organization of the mouse DPP IV gene. This gene, which encompasses more than 90 kb, is composed of 26 exons separated by introns, the lengths of which vary from 100 bp to more than 20 kb. Reverse PCR performed on RNA from different tissues indicated that DPP IV transcripts do not contain alternatively spliced CDS sequences and, therefore, are supposed to yield a single polypeptide. However, two types of specific mRNA have been detected that differ in their 3'UTR sequences. They derive from alternative polyadenylation of the DPP IV primary transcript, since the different 3'UTR sequences are contiguous in the mouse DPP IV gene. Sequence analysis of the gene 5'-flanking region revealed several structural features found in the TATAA-box-less promoters, including a G+C-rich segment, a high frequency of dinucleotide CpG, and an imperfect symmetrical dyad. The DPP IV gene was assigned by in situ hybridization to the mouse [2C2-2D] region, which is syntenic with human chromosome 2. These data indicate that the human Dpp4 locus is located within this synteny region (i.e., 2q14-q37). The genomic organization of the mouse DPP IV gene is compared to that of classical serine proteases and serine hydrolases. As structural and mechanistic conservation in the absence of sequence similarity is the most remarkable feature among alpha/beta hydrolases [Ollis, D. L., et al. (1992) Protein Eng. 5, 197-211], we report the possible evolutionary link between the DPP IV related family and alpha/beta hydrolases. |
| Databáze: | OpenAIRE |
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