Gene Transferin vitroandin vivowith Epstein-Barr Virus-Based Episomal Vector Results in Markedly High Transient Expression in Rodent Cells
| Autor: | Kazuhiro Nishizaki, Yohei Oda, Ki-ichiro Tomiyasu, Etsuko Satoh, Osam Mazda, Motoharu Kondo, Jiro Imanishi |
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| Rok vydání: | 1998 |
| Předmět: |
Herpesvirus 4
Human Genetic Vectors Biophysics Gene Expression Biology Transfection medicine.disease_cause Biochemistry Genome Marker gene Mice Plasmid hemic and lymphatic diseases Gene expression medicine Animals Humans Vector (molecular biology) Molecular Biology Gene Cells Cultured Myocardium Cell Biology Epstein–Barr virus Molecular biology Rats Muridae Plasmids |
| Zdroj: | Biochemical and Biophysical Research Communications. 253:733-738 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1998.9835 |
| Popis: | The EBV-based plasmid vectors, which carry oriP and EBNA1 gene from EBV genome, can be retained in the nucleus and replicate in human cells. Rodent cells are not permissive for the EBV plasmids, in terms of the plasmid replication. However, the EBV vector facilitates not only the long term maintenance of the plasmid but also high level gene expression at a transient phase after transfection. It has not been elucidated if rodent cells show this high level transient expression. We demonstrate that rodent cells transfected with an EBV vector expressed a marker gene more intensively than those with a conventional plasmid vector did. The high marker gene expression was also seen in rat myocardium injectedin vivowith the EBV plasmid. The present data indicate that the EBNA1-oriP system functions in “non-permissive” rodent cells at transient phase, and may require different cellular factor(s) for the transient expression and plasmid replication. |
| Databáze: | OpenAIRE |
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