Immunoglobulin heavy chain variable region gene utilization by B cell hybridomas derived from rheumatoid synovial tissue
| Autor: | C. M. S. Brown, A. Malcolm, C. Plater-Zyberk, C. Longhurst, G. Haynes, R. N. Maini |
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| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Adult
Immunology Immunoglobulin Variable Region chemical and pharmacologic phenomena Immunoglobulin E CD5 Antigens Arthritis Rheumatoid Antigen Antigens CD medicine Immunology and Allergy Humans B cell B-Lymphocytes Hybridomas biology Genes Immunoglobulin Synovial Membrane Middle Aged medicine.anatomical_structure biology.protein Immunoglobulin heavy chain Female Synovial membrane CD5 Antibody Clone (B-cell biology) Immunoglobulin Heavy Chains Research Article |
| Popis: | SUMMARY Rheumatoid arthritis (RA) is a chronic inflammatory disease that primarily affects synovial joints. Activated B lymphocytes and plasma cells are present in the synovial tissue and are thought to contribute to the immunopathology of the rheumatoid joint. To investigate rheumatoid synovial B lymphocytes, we have generated B cell hybridomas from synovial tissue of an RA patient. Here we describe the immunoglobulin VH gene repertoire of eight IgM- and 10 IgG-secreting synovial-derived hybridomas. The VH4 gene family is highly represented (38.5% in this panel of hybridomas compared with the frequeney of VH4 gene expression in circulating B lymphocytes reported previously (19-22%) and with the VH4 gene frequency we observed in a panel of hybridomas derived in the same manner from the spleen and tonsil of normal individuals (19%). The increased frequency of VH4 gene expression was not due to the expansion of a single B cell clone in vivo as none of these hybridomas was clonally related. Two synovial-derived hybridomas secreted autoantibodies; one (VH3+) secreted an IgM-rheumatoid factor (RF) and the other (VH4+) secreted IgM with polyreactive binding to cytoskeletal proteins and cardiolipin. The antibodies secreted by the remaining synovial-derived hybridomas were not reactive with the autoantigens tested. The VH gene usage in a proportion (5/17) of synovial-derived hybridomas that expressed CD5 antigen provided preliminary evidence that CD5+ B cells in RA synovium have a similar increase of VH4 gene expression reported for CD5+ B cells from normal individuals and patients with chronic lymphocytic leukaemia. |
| Databáze: | OpenAIRE |
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