Resveratrol inhibits glucose‐induced migration of vascular smooth muscle cells mediated by focal adhesion kinase
| Autor: | Ta Chun Yuan, Ping-Jyun Sung, Yi-Chen Chia, Thiyagarajan Varadharajan, Li-Hsuen Chen, May-Jywan Tsai, Yi‐Chiao Lin, Ching-Feng Weng |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Vascular smooth muscle Cell Survival MAP Kinase Signaling System Myocytes Smooth Muscle RAC1 CDC42 Matrix metalloproteinase Biology Resveratrol Microscopy Atomic Force Muscle Smooth Vascular CSK Tyrosine-Protein Kinase Focal adhesion Mice chemistry.chemical_compound Cell Movement Internal medicine Stilbenes medicine Animals Pseudopodia RNA Messenger Protein kinase A Cells Cultured Wound Healing Cell biology Mice Inbred C57BL Glucose src-Family Kinases Endocrinology Matrix Metalloproteinase 9 chemistry Focal Adhesion Protein-Tyrosine Kinases Matrix Metalloproteinase 2 Mitogen-Activated Protein Kinases Lamellipodium Food Science Biotechnology |
| Zdroj: | Molecular Nutrition & Food Research. 58:1389-1401 |
| ISSN: | 1613-4133 1613-4125 |
| DOI: | 10.1002/mnfr.201300698 |
| Popis: | Scope Diabetes is a critical factor for atherosclerosis, as hyperglycemia induces vascular smooth muscle cell (VSMC) proliferation and migration and subsequently contributes to the formation of atherosclerotic lesions. This study investigates whether resveratrol plays a regulatory role in the proliferation and migration of VSMCs under high glucose induction to imitate a hyperglycemic condition. Methods and results Resveratrol inhibited the migration of VSMCs in the wound-healing assay and the formation of lamellipodia and filopodia as assessed by atomic force microscopy scanning. Resveratrol suppressed the mRNA expression of c-Src, Rac1, cdc42, IRS-1, MEKK1, MEKK4, and mitogen-activated protein kinase along with the protein levels of c-Src, p-Src, and cdc42 in VSMCs. Resveratrol decreased the level of p-FAK protein under normal glucose conditions. Resveratrol could inhibit the activities of matrix metalloproteinase (MMP) 2 and MMP 9 as shown by zymography. Moreover, resveratrol also regulated the mitogen-activated protein kinase pathway and MMP activities of VSMC migration under the high glucose condition. Conclusion The antimigratory effects of resveratrol by reduced MMP expression through the inhibition of Rac1, p-FAK, and lamellipodia formation and the activation of p-AKT and p-ERK1/2 suggest that resveratrol is a potential compound for the treatment of vascular diseases via the regulation of VSMC migration. |
| Databáze: | OpenAIRE |
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