Tamoxifen decreases the myofibroblast count in the healing bile duct tissue of pigs
| Autor: | Angela Cristina Gouvêa Carvalho, Benedito Herani Filho, Andréa Rodrigues Cordovil Pires, José Mauro Granjeiro, Rafael Erthal de Paula, Antonio Claudio Lucas da Nóbrega, Orlando Hiroshi Kiono Siqueira, Nicolle Cavalcante Gaglionone, Fábio Otero Ascoli, Karin Soares Gonçalves Cunha |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty Swine Alpha (ethology) Cell Count Bile Duct Stricture Bile Duct Injury medicine Animals Myofibroblasts Wound Healing lcsh:R5-920 Bile duct business.industry Estrogen Antagonists Reproducibility of Results Muscle Smooth General Medicine medicine.disease Immunohistochemistry Actins Stenosis Tamoxifen medicine.anatomical_structure Basic Research Treatment Outcome Biliary tract Female Bile Ducts business Biliary Wound Healing lcsh:Medicine (General) Myofibroblast Immunostaining medicine.drug |
| Zdroj: | Clinics, Vol 68, Iss 1, Pp 101-106 (2013) Clinics; v. 68 n. 1 (2013); 101-106 Clinics; Vol. 68 Núm. 1 (2013); 101-106 Clinics; Vol. 68 No. 1 (2013); 101-106 Clinics Universidade de São Paulo (USP) instacron:USP Clinics, Volume: 68, Issue: 1, Pages: 101-106, Published: JAN 2013 |
| ISSN: | 1980-5322 1807-5932 |
| Popis: | OBJECTIVE: The aim of this study was to evaluate the effect of oral tamoxifen treatment on the number of myofibroblasts present during the healing process after experimental bile duct injury. METHODS: The sample consisted of 16 pigs that were divided into two groups (the control and study groups). Incisions and suturing of the bile ducts were performed in the two groups. Tamoxifen (20 mg/day) was administered only to the study group. The animals were sacrificed after 30 days. Quantification of myofibroblasts in the biliary ducts was made through immunohistochemistry analysis using anti-alpha smooth muscle actin of the smooth muscle antibody. Immunohistochemical quantification was performed using a digital image system. RESULTS: In the animals treated with tamoxifen (20 mg/day), there was a significant reduction in immunostaining for alpha smooth muscle actin compared with the control group (0.1155 vs. 0.2021, p = 0.046). CONCLUSION: Tamoxifen reduced the expression of alpha smooth muscle actin in the healing tissue after bile duct injury, suggesting a decrease in myofibroblasts in the scarred area of the pig biliary tract. These data suggest that tamoxifen could be used in the prevention of biliary tract stenosis after bile duct surgeries. |
| Databáze: | OpenAIRE |
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