Molecular epidemiological analysis of wild animal rabies isolates from India
| Autor: | S. Vineetha, Rajendra Singh, Anil Kumar Sharma, Mani Saminathan, Gundallhalli Bayyappa Manjunatha Reddy, Basavaraj Sajjanar, Karam Pal Singh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Veterinary medicine 030106 microbiology India medicine.disease_cause SF1-1100 03 medical and health sciences Genotype wild animals SF600-1100 medicine rabies virus Genetic variability nucleoprotein gene Direct fluorescent antibody Lyssavirus General Veterinary biology Phylogenetic tree phylogenetic analysis Rabies virus biology.organism_classification medicine.disease Virology Animal culture Reverse transcription polymerase chain reaction 030104 developmental biology Rabies Research Article |
| Zdroj: | Veterinary World, Vol 12, Iss 3, Pp 352-357 (2019) Veterinary World |
| ISSN: | 2231-0916 0972-8988 |
| Popis: | Aim: This study was conducted to know the genetic variability of rabies viruses (RVs) from wild animals in India. Materials and Methods: A total of 20 rabies suspected brain samples of wild animals from different states of India were included in the study. The samples were subjected for direct fluorescent antibody test (dFAT), reverse transcription polymerase chain reaction (RT-PCR), and quantitative reverse transcriptase real-time PCR (RT-qPCR). The phylogenetic analysis of partial nucleoprotein gene sequences was performed. Results: Of 20 samples, 11, 10, and 12 cases were found positive by dFAT, RT-PCR, and RT-qPCR, respectively. Phylogenetic analysis showed that all Indian wild RVs isolates belonged to classical genotype 1 of Lyssavirus and were closely related to Arctic/Arctic-like single cluster indicating the possibility of a spillover of rabies among different species. Conclusion: The results indicated the circulation of similar RVs in sylvatic and urban cycles in India. However, understanding the role of wild animals as reservoir host needs to be studied in India. |
| Databáze: | OpenAIRE |
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