The application of novel 1.7 μm ethylene bridged hybrid particles for hydrophilic interaction chromatography

Autor:	Diane M. Diehl, Eric S. Grumbach, Uwe D. Neue
Rok vydání:	2008
Předmět:	Chemical resistance Electrospray Chromatography Ethylene Chemistry Hydrophilic interaction chromatography Analytical chemistry Filtration and Separation Analytical Chemistry chemistry.chemical_compound Phase (matter) Particle Chemical stability Particle size
Zdroj:	Journal of Separation Science. 31:1511-1518
ISSN:	1615-9314 1615-9306
DOI:	10.1002/jssc.200700673
Popis:	An un-derivatized 1.7 microm ethylene bridged hybrid (BEH) particle was evaluated for its utility in retaining polar species in hydrophilic interaction chromatography (HILIC), and was compared to a 3 microm un-derivatized silica material. Retentivity as a function of mobile phase pH, polar modifier and ACN content was examined. Also, the efficiency of the two particle substrates was compared by plotting HETP vs. linear velocity. Improved chemical resistance of the un-derivatized BEH particle was compared to un-derivatized silica at pH 5, demonstrating no performance deterioration over the course of 2000 injections for the BEH particle, while the silica particle deteriorated rapidly after 800 injections. Lastly, ESI-MS sensitivity as a function of particle size and separation mode was demonstrated. A 2.2 to 4.7-times higher ESI-MS response was observed on the 1.7 microm particle compared to the 3 microm particle, whereas a 5.6 to 8.8-times higher ESI-MS response was observed using HILIC as when compared to traditional RP chromatography.
Databáze:	OpenAIRE
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