MicroRNA-214-5p protects against myocardial ischemia reperfusion injury through targeting the FAS ligand
| Autor: | Fengyun Zhang, Zhirong Wang, Yao Zhang, Xiaoqin Hu, Jue Xi, Li Zhi, Min Zhang, Chenzong Li, Wensu Chen, Hui Wei, Yuan Lu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species ischemic heart diseases cell apoptosis business.industry General Medicine medicine.disease Molecular biology FASLG In vitro Fas ligand 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Basic Research chemistry Apoptosis In vivo Lactate dehydrogenase medicine 030212 general & internal medicine miR-214-5p business Cell damage Reperfusion injury |
| Zdroj: | Archives of Medical Science : AMS |
| ISSN: | 1896-9151 1734-1922 |
| Popis: | Introduction MicroRNAs (miRNAs) are considered as crucial modulators in myocardial ischemia and reperfusion (I/R) injury. The present study aimed to investigate the expression and biological functions of miR-214-5p via targeting Fas ligand (FASLG) in I/R injury. Material and methods Lactate dehydrogenase, casein kinase, malondialdehyde assay, reactive oxygen species (ROS) detection and cell apoptosis analysis measured cell damage and cell apoptosis in H9c2 cells under hypoxia/reperfusion (H/R) treatment. Bioinformatics and dual luciferase reporter assays demonstrated the molecular mechanism of miR-214-5p in cardiac cells. 2,3,5-Triphenyltetrazolium chloride (TTC) staining, hematoxylin-eosin (HE) staining and adenovirus injection were performed in I/R treated mice. Results The expression of miR-214-5p was decreased in H/R injured H9c2 cells compared with control cells (p < 0.001). Overexpression of miR-214-5p reduced cell damage and apoptosis in H9c2 cells under H/R treatment (p < 0.001). Further study revealed that FASLG was a target of miR-214-5p. Enhanced expression of FASLG attenuated the protective function of miR-214-5p in H9c2 cells subjected to H/R injury (P < 0.001). Moreover, the elevated expression of miR-214-5p by adenovirus injection protected cardiac cells from I/R injury in mice (n = 6/per group). Conclusions We found that miR-214-5p exerted a protective role in I/R injured cardiac cells by direct targeting FASLG in vitro and in vivo. |
| Databáze: | OpenAIRE |
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