Localization and polymorphism of a chromosome 12-specific α satellite DNA sequence
| Autor: | Vincent T.H.B.M. Smit, J.W. Oosterhuis, C.J. Cornelisse, Leendert H. J. Looijenga, P. Mollevanger, Peter Devilee, J.W. Wessels |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Genetics
Chromosomes Human Pair 12 biology Marker chromosome Centromere Chromosome Mapping Nucleic Acid Hybridization DNA Satellite HindIII Molecular biology Restriction enzyme Genetic marker biology.protein Humans Restriction fragment length polymorphism DNA Probes Molecular Biology Chromosome 22 Polymorphism Restriction Fragment Length Genetics (clinical) Chromosome 12 Southern blot |
| Zdroj: | Cytogenetic and Genome Research. 53:216-218 |
| ISSN: | 1424-859X 1424-8581 |
| Popis: | The isolation and localization of a chromosome 12-specific α satellite DNA sequence, pα12H8, is described. This clone contains a complete copy of the 1.4-kb Hindlll higher-order repeat present within the α satellite array on chromosome 12. The specificity of pα12H8 was demonstrated by in situ hybridization and Southern blot analysis of a somatic cell hybrid mapping panel, both performed under high-stringency conditions. Polymorphic restriction patterns within the α satellite array, revealed by the use of the restriction enzymes Bglll and EcoRV, were demonstrated to display Mendelian inheritance. These properties make pα12H8 a valuable genetic marker for the centromeric region of chromosome 12. |
| Databáze: | OpenAIRE |
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