Modulation of integrin expression on rat bone marrow cells by substrates with different surface characteristics
| Autor: | P.J. ter Brugge, John A. Jansen, Ruurd Torensma, Carl G. Figdor, J.E. de Ruijter |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Calcium Phosphates
Integrins Surface Properties Protein subunit Integrin Implantology and biomaterials Cell Culture Techniques chemistry.chemical_element Bone Marrow Cells Cell Count Rat Bone Marrow Calcium medicine Animals Trypsin Rats Wistar Cells Cultured Titanium biology Chemistry urogenital system General Engineering Rats Cell biology Integrin expression Cell culture Immunology embryonic structures biology.protein Polystyrenes Implantologie en biomaterialen Tumorimmunology medicine.drug |
| Zdroj: | Tissue Engineering, 8, 615-26 Tissue Engineering, 8, 615-626. Mary ann liebert inc publ Tissue Engineering, 8, 4, pp. 615-26 Tissue Engineering, 8, 4, pp. 615-626 |
| ISSN: | 1076-3279 2152-4947 |
| Popis: | Item does not contain fulltext Biomaterials have been shown to be able to influence the growth and differentiation of osteogenic cells cultured on the surface. Although the precise mechanisms by which the materials influence osteogenic cells are unclear, it is possible that the materials manipulate the expression of integrins by the cells. We therefore studied the expression of a number of integrins by rat bone marrow (RBM) cells, after culture on culture polystyrene, on machined and grit-blasted titanium, and on calcium phosphate-coated titanium. Integrin expression was studied by FACS analysis. We found a large variation in the expression of integrins by cells in replicate experiments. After culture on polystyrene for 7 days, cells expressed alpha1, alpha2, alpha3, alpha5, alpha6, beta1, and beta3, although some of the subunits were expressed only occasionally. The cells did not express the alpha4 subunit. After culture of RBM cells for 8 days on coated and noncoated titanium substrates, cells always expressed alpha3, alpha5, alpha6, and beta1. The alpha1 and beta3 subunits were only expressed in some of the experiments. Frequently, the expression of alpha5, alpha6, and beta1 was higher on the coated than on the noncoated titanium substrates. Based on our results, we conclude that the studied materials are capable of influencing the expression of integrins by RBM cells cultured on relevant implant materials. |
| Databáze: | OpenAIRE |
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