Low immunogenicity of in vitro-expanded human neural cells despite high MHC expression
| Autor: | Elisabet Åkesson, Scott Falci, Jing-Hua Piao, Eva-Britt Samuelsson, Jenny Odeberg |
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| Rok vydání: | 2005 |
| Předmět: |
Time Factors
Immunology Cell Count Human leukocyte antigen Lymphocyte Activation Major histocompatibility complex Major Histocompatibility Complex Fetus Prosencephalon Antigens CD Pregnancy Neurosphere Humans Immunology and Allergy Cells Cultured Cell Proliferation Neurons CD86 Analysis of Variance CD40 biology Stem Cells Immunogenicity Gene Expression Regulation Developmental Embryo Mammalian Flow Cytometry Immunohistochemistry Coculture Techniques In vitro Cell biology Spinal Cord Neurology biology.protein Female Neurology (clinical) CD80 |
| Zdroj: | Journal of Neuroimmunology. 161:1-11 |
| ISSN: | 0165-5728 |
| Popis: | The ability to expand human neural precursor cells in vitro offers new possibilities for future cell therapies. However, concern over immunologically based rejection of in vitro-expanded human neural cells confounds their use as donor cells. Here, we demonstrate that the expression of human leukocyte antigen (HLA) class I and II molecules, but not the co-stimulatory proteins CD40, CD80 and CD86, substantially increase during expansion of neurospheres. Furthermore, peripheral lymphocytes were unresponsive when co-cultured with in vitro-expanded neural cells. Taken together, these results suggest a low immunogenicity of these cultured human neural cells despite HLA incompatibility and high HLA expression. |
| Databáze: | OpenAIRE |
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