Live-Cell Surface-Enhanced Raman Spectroscopy Imaging of Intracellular pH: From Two Dimensions to Three Dimensions
| Autor: | Yizhi Zhang, Malou Henriksen-Lacey, Dorleta Jimenez de Aberasturi, Judith Langer, Luis M. Liz-Marzán |
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| Rok vydání: | 2020 |
| Předmět: |
Intracellular pH
Metal Nanoparticles Nanoparticle Bioengineering 02 engineering and technology Spectrum Analysis Raman Endocytosis 01 natural sciences symbols.namesake Live cell imaging Instrumentation Plasmon Fluid Flow and Transfer Processes Chemistry Process Chemistry and Technology 010401 analytical chemistry Hydrogen-Ion Concentration Surface-enhanced Raman spectroscopy 021001 nanoscience & nanotechnology 0104 chemical sciences Biophysics symbols Gold 0210 nano-technology Raman spectroscopy Intracellular |
| Zdroj: | ACS Sensors |
| Popis: | Visualization of intracellular pH (i-pH) using surface-enhanced Raman spectroscopy (SERS) plays an important role toward understanding of cellular processes including their interactions with nanoparticles. However, conventional two-dimensional SERS imaging often fails to take into consideration changes occurring in the whole-cell volume. We therefore aimed at obtaining a comprehensive i-pH profile of living cells by means of three-dimensional (3D) SERS imaging, thereby visualizing dynamic i-pH distribution changes in a single cell. We devised here a biocompatible and highly stable SERS pH probe, comprising plasmonic gold nanostars functionalized with a pH-sensitive Raman reporter tag—4-mercaptobenzoic acid—and protected by a cationic biocompatible polymer, poly-l-arginine hydrochloride (PA). The positively charged PA coating plays a double role in enhancing cell uptake and providing chemical and colloidal stability in cellular environments. The SERS-active pH probe allowed visualization of local changes in i-pH, such as acidification during nanoparticle (NP) endocytosis. We provide evidence of i-pH changes during NP endocytosis via high-resolution 3D SERS imaging, thereby opening new avenues toward the application of SERS to intracellular studies. |
| Databáze: | OpenAIRE |
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