Collagen Characterization in a Model of Nonalcoholic Steatohepatitis with Fibrosis; A Call for Development of Targeted Therapeutics
| Autor: | Itzhak D. Goldberg, Kiera Spahn, Prakash Narayan, Ping Zhou, Anthony J Pellicano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Nonalcoholic steatohepatitis
Male Pathology Pharmaceutical Science Organic chemistry Analytical Chemistry Transcriptome collagen type III chemistry.chemical_compound Liver disease Collagen Type III Mice 0302 clinical medicine QD241-441 Fibrosis Non-alcoholic Fatty Liver Disease Drug Discovery Nonalcoholic fatty liver disease NASH Up-Regulation precision therapeutics Liver Chemistry (miscellaneous) 030220 oncology & carcinogenesis Molecular Medicine 030211 gastroenterology & hepatology Female Collagen Microscopy Polarization medicine.medical_specialty collagen type I digestive system Article 03 medical and health sciences Hydroxyproline Parenchyma medicine Animals Physical and Theoretical Chemistry business.industry Gene Expression Profiling fibrosis transcriptomic medicine.disease digestive system diseases Collagen Type I alpha 1 Chain Disease Models Animal chemistry Gene Expression Regulation Case-Control Studies Fast Foods business |
| Zdroj: | Molecules Molecules, Vol 26, Iss 3316, p 3316 (2021) |
| ISSN: | 1420-3049 |
| Popis: | Left untreated, nonalcoholic fatty liver disease can progress to nonalcoholic steatohepatitis (NASH), fibrosis, and end-stage liver disease. To date, few if any therapies have proven effective against NASH with fibrosis. Quantification and qualification of hepatic scar might enable development of more effective targeted therapies. In a murine model of NASH induced by diet, we characterized fibrillar collagen deposition within the hepatic parenchyma. At harvest, livers from the modified diet cohort exhibited NASH with fibrosis. Transcriptomic analysis of hepatic tissue revealed increased col1a1, col1a2, and col3a1, each of which correlated directly with hepatic hydroxyproline content. Circular polarized microscopic analysis of Picrosirius red-stained liver sections revealed deposition of collagen type I within the parenchyma. Development of therapeutics designed to mitigate collagen type I accumulation might prove effective in NASH with fibrosis. |
| Databáze: | OpenAIRE |
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