Engineering and monitoring cellular barrier models
| Autor: | Mar Álvarez, Xavi Illa, Rosa Villa, J. Yeste |
|---|---|
| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Centro de Investigación Biomédica en Red Bioingeniería, Biomateriales y Nanomedicina (España) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Environmental Engineering Chemistry Cell barrier function Biomedical Engineering Review 02 engineering and technology Cell Biology 021001 nanoscience & nanotechnology Cell Microenvironment 03 medical and health sciences Multicellular organism Transepithelial electrical properties 030104 developmental biology lcsh:Biology (General) Nucleic acid chemistry Microphysiological systems Biological barriers Barrier permeability 0210 nano-technology lcsh:QH301-705.5 Molecular Biology Neuroscience Barrier function |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Journal of Biological Engineering Journal of Biological Engineering, Vol 12, Iss 1, Pp 1-19 (2018) |
| ISSN: | 1754-1611 |
| Popis: | Epithelia and endothelia delineate tissue compartments and control their environments by regulating the passage of ions and solutes. This barrier function is essential for the development and maintenance of multicellular organisms, and its dysfunction is associated with numerous human diseases. Recent advances in biomaterials and microfabrication technologies have evolved in vitro approaches for modelling biological barriers. Current microphysiological systems have become more efficient and reliable in mimicking the cell microenvironment. Additionally, methods for the quantification of barrier permeability have long provided significant insight into their underlying mechanisms. In this review, we outline the current techniques to quantify the barrier function of engineered tissues, and we also give an overview of recent microphysiological systems of biological barriers that emulate the microenvironment and microarchitecture of native tissues. This work has been supported by grants from the Ministerio de Economía y Competitividad (MINECO) (SAF2014–62114-EXP, DPI2015–65401-C3–3-R, and RYC-2013-14479) and from CIBER in Bioengineering, Biomaterials & Nanomedicine (CIBER-BBN). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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