Identification of the infectious source of an unusual outbreak of histoplasmosis, in a hotel in Acapulco, state of Guerrero, Mexico
| Autor: | Roberto Suárez-Alvarez, Maria Lucia Taylor, Aurora Hernández-Ramírez, José Sifuentes-Osornio, Jorge H. Sahaza-Cardona, Laura E. Carreto-Binaghi, María del Rocío Reyes-Montes, Luis E. Soto-Ramírez, Yuri A. Roldán-Aragón, Gabriela Rodríguez-Arellanes, Guillermo M. Ruiz-Palacios, Armando Pérez, Esperanza Duarte-Escalante, Gabriela R. Peña-Sandoval, Rafael Romero-Martinez |
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| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
Sequence analysis Histoplasma Immunology Enzyme-Linked Immunosorbent Assay Biology Polymerase Chain Reaction complex mixtures Microbiology Histoplasmosis Disease Outbreaks law.invention Fungal Proteins Mice law medicine Animals Humans Immunology and Allergy Mexico Gene Soil Microbiology Polymerase chain reaction Mice Inbred BALB C Travel Inoculation Outbreak Sequence Analysis DNA General Medicine medicine.disease Virology Random Amplified Polymorphic DNA Technique RAPD Infectious Diseases Organ Specificity Nested polymerase chain reaction |
| Zdroj: | FEMS Immunology & Medical Microbiology. 45:435-441 |
| ISSN: | 1574-695X 0928-8244 |
| Popis: | Three isolates of Histoplasma capsulatum were identified from mice lung, liver, and spleen inoculated with soil samples of the X hotel's ornamental potted plants that had been fertilized with organic material known as compost. The presence of H. capsulatum in the original compost was detected using the dot-enzyme-linked immunosorbent assay. Nested-PCR, using a specific protein Hcp100 coding gene sequence, confirmed the fungal identification associated with an unusual histoplasmosis outbreak in Acapulco. Although, diversity between the H. capsulatum isolate from the hotel and some clinical isolates from Guerrero (positive controls) was observed using random amplification of polymorphic DNA based-PCR, sequence analyses of H-anti and ole fragment genes revealed a high homology (92-99%) between them. |
| Databáze: | OpenAIRE |
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