A Novel Function of the Lysophosphatidic Acid Receptor 3 (LPAR3) Gene in Zebrafish on Modulating Anxiety, Circadian Rhythm Locomotor Activity, and Short-Term Memory

Autor: Hsinyu Lee, Jen Her Lu, Chung-Der Hsiao, Gilbert Audira, Nemi Malhotra, Nguyen Thi Ngoc Anh, Petrus Siregar, Yu Nung Lin
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Anxiety
lcsh:Chemistry
memory
Animals
Genetically Modified
chemistry.chemical_compound
Gene Knockout Techniques
Lysophosphatidic acid
Receptors
Lysophosphatidic Acid
Receptor
Zebrafish
lcsh:QH301-705.5
Spectroscopy
Neurotransmitter Agents
Principal Component Analysis
Color Perception Tests
lysophosphatidic acid receptor
Brain
General Medicine
Computer Science Applications
Cell biology
Circadian Rhythm
Aggression
Memory
Short-Term
Multigene Family
Lysophosphatidic Acid Receptor 3
Locomotion
circadian rhythm locomotor activity
Enzyme-Linked Immunosorbent Assay
Biology
Catalysis
Article
Inorganic Chemistry
Avoidance Learning
Animals
Circadian rhythm
Physical and Theoretical Chemistry
Molecular Biology
LPAR3
LPAR1
behavior
LPAR6
Organic Chemistry
biology.organism_classification
Hormones
chemistry
lcsh:Biology (General)
lcsh:QD1-999
Gene Expression Regulation
Behavior Rating Scale
Exploratory Behavior
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 8
International Journal of Molecular Sciences, Vol 21, Iss 2837, p 2837 (2020)
ISSN: 1422-0067
Popis: Lysophosphatidic acid (LPA) is a small lysophospholipid molecule that activates multiple cellular functions through pathways with G-protein-coupled receptors. So far, six LPA receptors (LPAR1 to LPAR6) have been discovered and each one of them can connect to the downstream cell message-transmitting network. A previous study demonstrated that LPA receptors found in blood-producing stem cells can enhance erythropoietic processes through the activation of LPAR3. In the current study, newly discovered functions of LPAR3 were identified through extensive behavioral tests in lpar3 knockout (KO) zebrafish. It was found that the adult lpar3 KO zebrafish display an abnormal movement orientation and altered exploratory behavior compared to that of the control group in the three-dimensional locomotor and novel tank tests, respectively. Furthermore, consistent with those results, in the circadian rhythm locomotor activity test, the lpar3 KO zebrafish showed a lower level of angular velocity and average speed during the light cycles, indicating an hyperactivity-like behavior. In addition, the mutant fish also exhibited considerably higher locomotor activity during the dark cycle. Supporting those findings, this phenomenon was also displayed in the lpar3 KO zebrafish larvae. Furthermore, several important behavior alterations were also observed in the adult lpar3 KO fish, including a lower degree of aggression, less interest in conspecific social interaction, and looser shoal formation. However, there was no significant difference regarding the predator avoidance behavior between the mutant and the control fish. In addition, lpar3 KO zebrafish displayed memory deficiency in the passive avoidance test. These in vivo results support for the first time that the lpar3 gene plays a novel role in modulating behaviors of anxiety, aggression, social interaction, circadian rhythm locomotor activity, and memory retention in zebrafish.
Databáze: OpenAIRE
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