The expression of antioxidant enzymes in the gingivae of type 2 diabetics with chronic periodontitis
| Autor: | Poliana Mendes Duarte, Marta Ferreira Bastos, Vera Cavalcanti de Araújo, Ana Paula Dias Demasi, V. R. Santos, Ellen C. Fagnani, Fernancla V. Ribeiro, Marcelo Henrique Napimoga |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male medicine.medical_specialty GPX1 Biopsy Gingiva SOD2 Gene Expression Polymerase Chain Reaction Antioxidants Superoxide dismutase Internal medicine Diabetes mellitus medicine Humans RNA Messenger General Dentistry chemistry.chemical_classification Periodontitis Glutathione Peroxidase biology Dentistry(all) Superoxide Dismutase business.industry Glutathione peroxidase Peroxiredoxins Cell Biology General Medicine Middle Aged Catalase medicine.disease Chronic periodontitis Oxidative Stress Endocrinology Diabetes Mellitus Type 2 Otorhinolaryngology chemistry Case-Control Studies Chronic Periodontitis Immunology biology.protein Female business Peroxiredoxin |
| Zdroj: | Archives of Oral Biology. 57:161-168 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/j.archoralbio.2011.08.007 |
| Popis: | There is controversial evidence regarding the levels of antioxidant molecules in type 2 diabetes periodontitis patients. Thus, the aim of the present study was to evaluate the gene expression of antioxidant enzymes in the gingival tissue of poorly and well-controlled type 2 diabetic subjects with chronic periodontitis (CP).Gingival biopsies were harvested from systemically and periodontally healthy subjects (n=12), systemically healthy subjects with CP (n=15), well-controlled (n=8) and poorly controlled (n=14) diabetic subjects with CP. The messenger RNA (mRNA) levels of peroxiredoxin (PRDX) 1 and 2, catalase (CAT), glutathione peroxidase (GPX1) and superoxide dismutase (SOD) 1 and 2 were measured by quantitative polymerase chain reaction (qPCR).The results showed that PRDX1 and GPX1 were up-regulated by periodontitis (p0.05), independently of the glycaemic status, whilst PRDX2 and SOD2 genes were slightly influenced by periodontitis, but significantly induced when periodontitis was associated with DM, especially under a poor glycaemic control (p0.05). Moreover, CAT and SOD1 expressions were not significantly influenced by any of these inflammatory disorders (p0.05).In conclusion, both PRDX1 and GPX1 were overexpressed in CP whilst PRDX2 and SOD2 were up-regulated especially in the poorly controlled diabetic group with CP. |
| Databáze: | OpenAIRE |
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