Fertilization of C57BL/6 mouse sperm collected from cauda epididymides after preservation or transportation at 4 degrees C using laser-microdissected oocytes
| Autor: | Yukie Haruguchi, Hiromi Machida, Shuuji Tsuchiyama, T. Kondo, Naomi Nakagata, Kiyoko Fukumoto, Takehito Kaneko, Shiho Noshiba, Mika Koga, Kiyora Saiki, Yoshiko Nakagawa |
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| Rok vydání: | 2008 |
| Předmět: |
Male
endocrine system medicine.medical_treatment Fertilization in Vitro Biology Models Biological General Biochemistry Genetics and Molecular Biology Cryopreservation Andrology Mice Human fertilization Freezing medicine Animals Zona pellucida reproductive and urinary physiology Sperm plasma membrane Epididymis In vitro fertilisation urogenital system Lasers Cell Membrane Embryo General Medicine Sperm Spermatozoa Mice Inbred C57BL medicine.anatomical_structure Fertilization Oocytes Female General Agricultural and Biological Sciences |
| Zdroj: | Cryobiology. 59(1) |
| ISSN: | 1090-2392 |
| Popis: | The C57BL/6 mouse is commonly used to produce transgenic and knockout strains for biomedical research. However, the motility and fertility of its sperm decrease markedly with freezing. Short-term preservation of sperm without freezing can avoid this. Furthermore, such samples can be transported safety without the special skills or equipment needed for the transportation of live animals or frozen products. We evaluated the motility and fertility of sperm collected from cauda epididymides after preservation or transportation at 4 degrees C. Oocytes with the zona pellucida subjected to laser-microdissection were used to assist fertilization in vitro. Although the motility of sperm gradually decreased with storage (P |
| Databáze: | OpenAIRE |
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