Calcium in smooth muscle sarcoplasmic reticulum in situ. Conventional and X-ray analytical electron microscopy
| Autor: | I Diculescu, L M Popescu |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
In situ
Colon Guinea Pigs chemistry.chemical_element Calcium Biology Oxalate chemistry.chemical_compound medicine Animals Magnesium Oxalates Endoplasmic reticulum X-ray Muscle Smooth Depolarization Articles Cell Biology Osmium Taenia coli Calcium ATPase Sarcoplasmic Reticulum Zinc medicine.anatomical_structure chemistry Biochemistry Potassium Biophysics Chlorine Copper |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.67.3.911 |
| Popis: | Numerous electron-opaque deposits appear in the SR of the relaxed smooth muscle cells of the guinea pig Taenia coli that had been treated, before fixation, with a depolarizing medium containing oxalate to precipitate calcium ions in situ. X-ray spectra obtained by spot and line-scanning analyses of these deposits in situ show characteristic calcium signals, thus providing direct evidence for calcium accumulation inside the sarcoplasmic reticulum of smooth muscle. |
| Databáze: | OpenAIRE |
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