Structure of neuro-endocrine and neuro-epithelial interactions in human foetal pancreas
| Autor: | Sergey Saveliev, Alexandra Proshchina, Yuliya Krivova, O. G. Leonova, V. M. Barabanov |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Nervous system medicine.medical_specialty Gestational Age Enteroendocrine cell Biology Nervous System Glucagon S100 protein 03 medical and health sciences Cytokeratin Fetus Tubulin Internal medicine medicine Humans Insulin Endocrine system Pancreas Keratin-19 S100 Proteins Gene Expression Regulation Developmental Epithelial Cells Cell Biology General Medicine Cell biology 030104 developmental biology medicine.anatomical_structure Endocrinology Immunohistochemistry Ganglia Endocrine Cells Developmental Biology |
| Zdroj: | Tissue and Cell. 48:567-576 |
| ISSN: | 0040-8166 |
| DOI: | 10.1016/j.tice.2016.10.005 |
| Popis: | In the pancreas of many mammals including humans, endocrine islet cells can be integrated with the nervous system components into neuro-insular complexes. The mechanism of the formation of such complexes is not clearly understood. The present study evaluated the interactions between the nervous system components, epithelial cells and endocrine cells in the human pancreas. Foetal pancreas, gestational age 19–23 weeks (13 cases) and 30–34 weeks (7 cases), were studied using double immunohistochemical labeling with neural markers (S100 protein and beta III tubulin), epithelial marker (cytokeratin 19 (CK19)) and antibodies to insulin and glucagon. We first analyse the structure of neuro-insular complexes using confocal microscopy and provide immunohistochemical evidences of the presence of endocrine cells within the ganglia or inside the nerve bundles. We showed that the nervous system components contact with the epithelial cells located in ducts or in clusters outside the ductal epithelium and form complexes with separate epithelial cells. We observed CK19-positive cells inside the ganglia and nerve bundles which were located separately or were integrated with the islets. Therefore, we conclude that neuro-insular complexes may forms as a result of integration between epithelial cells and nervous system components at the initial stages of islets formation. |
| Databáze: | OpenAIRE |
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