Enhanced antitumor activity of epigallocatechin gallate-conjugated dual-drug-loaded polystyrene-polysoyaoil-diethanol amine nanoparticles for breast cancer therapy
| Autor: | Zeynep Karahaliloğlu, Pınar Alpaslan, Baki Hazer, Emir Baki Denkbaş, Ebru Kilicay |
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| Přispěvatelé: | [Karahaliloglu, Zeynep] Aksaray Univ, Div Biol, TR-68000 Aksaray, Turkey -- [Kilicay, Ebru] Bulent Ecevit Univ, Elect & Automat Div, Zonguldak, Turkey -- [Alpaslan, Pinar] TOBB Univ Econ & Technol, Dept Biomed Engn, Ankara, Turkey -- [Hazer, Baki] Bulent Ecevit Univ, Div Phys Chem, Zonguldak, Turkey -- [Denkbas, Emir Baki] Hacettepe Univ, Div Biochem, Ankara, Turkey, TOBB ETÜ, Mühendislik Fakültesi, Biyomedikal Mühendisliği Bölümü, TOBB ETU, Faculty of Engineering, Department of Biomedical Engineering, Alpaslan, Pınar, Alpaslan, Pınar [57195150399], Sabire Yazıcı Fen Edebiyat Fakültesi, Zonguldak Bülent Ecevit Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Drug
α-tocopheryl succinate epigallocatechin gallate Curcumin Polymers and Plastics media_common.quotation_subject α-Tocopheryl Succinate Nanoparticle Bioengineering 02 engineering and technology Conjugated system Epigallocatechin gallate polystyrene Biomaterials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine soya oil ?-tocopheryl succinate Materials Chemistry tocopheryl succinate Soya oil Polystyrene media_common Chemistry 021001 nanoscience & nanotechnology Combinatorial chemistry Bioavailability 030220 oncology & carcinogenesis Drug delivery drug delivery Amine gas treating 0210 nano-technology |
| Popis: | WOS: 000418857200003 The development of novel combination anticancer drug delivery systems is an important step to improve the effectiveness of anticancer treatment in metastatic breast cancer and to overcome increased toxicity of the currently used combination treatments. The aim of this study was to assess efficient targeting, therapeutic efficacy, and bioavailability of a combination of drugs (curcumin and -tocopheryl succinate) loaded polystyrene-polysoyaoil-diethanol amine nanoparticles. Polystyrene-polysoyaoil-diethanol amine nanoparticles encapsulating two drugs, individually or in combination, were prepared by double-emulsion solvent evaporation method, resulting in particle size smaller than 250nm with a surface negative charge between -30 and -40mV. Entrapment efficiency of curcumin and -tocopheryl succinate in the epigallocatechin gallate-conjugated dual-drug-loaded nanoparticles was found to be 68% and 80%, respectively. The release kinetics of curcumin and -tocopheryl succinate from the nanoparticles exhibited a gradual and continuous profile followed by an initial burst behavior with a release over 20days in vitro. Next, we have investigated the anticancer activity of nanoparticles encapsulating both the drugs and individually drug in human breast cancer cells (MDA-MB-231) using double-staining-based cell death analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assessment of cytotoxicity and flow cytometer. In vitro cytotoxicity studies revealed that epigallocatechin gallate--tocopheryl succinate/curcumin-polystyrene-polysoyaoil-diethanol amine nanoparticles are more potent than the corresponding -tocopheryl succinate/curcumin-polystyrene-polysoyaoil-diethanol amine nanoparticles and their single-drug-loaded forms and show a synergistic and breast tumor targeting function. Thus, here, we propose epigallocatechin gallate-conjugated curcumin and -tocopheryl succinate-loaded polystyrene-polysoyaoil-diethanol amine nanoparticles which effectively inhibit tumor growth and reduce toxicity compared to single-drug chemotherapy. Aksaray UniversityAksaray University [2016-028]; Bulent Ecevit UniversityBulent Ecevit University [BEU-2015-72118496-12] This work was financially supported by Aksaray University (project no. 2016-028) and Bulent Ecevit University (BEU-2015-72118496-12). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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