Analysis of wheat mitochondrially encoded polypeptides by in organello labelling

Autor:	J. M. Grienenberger, B. Combettes
Rok vydání:	2019
Předmět:	Immunoprecipitation Respiratory chain food and beverages Embryo Plant Science General Medicine Mitochondrion Biology Molecular biology Respiratory chain complex I Blot Biochemistry Labelling Etiolation Agronomy and Crop Science
Zdroj:	Plant cell reports. 19(2)
ISSN:	1432-203X
Popis:	The in organello labeling pattern in wheat (Triticum aestivum) mitochondria isolated from imbibed embryos were compared with those from the commonly used starting material, etiolated seedlings. Mitochondria from imbibed embryos proved to be metabolically more active than those from etiolated seedlings and produced a large number of strongly in organello-labeled polypeptides. Immunoprecipitation of the labeled proteins enabled the identification of mitochondrially encoded subunits of the respiratory chain complex I, some of which could not be detected by conventional Western blotting due to their high hydrophobicity. A method for mass isolation of wheat embryos is also presented which allows easy preparation of large amounts of intact and highly active mitochondria suitable for biochemical studies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b71f01ae1b6d4f4e8591fc1bed2fed54 https://pubmed.ncbi.nlm.nih.gov/30754742 Zobrazit plný text záznamu Full text from SpringerLink