Evaluation of the cloned enzyme donor immunoassay for measurement of phenytoin and phenobarbital in serum
| Autor: | A H Veefkind, J van der Weide, H J Luiting |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Phenytoin
medicine.medical_treatment Cross Reactions High-performance liquid chromatography Immunoenzyme Techniques medicine Humans Pharmacology (medical) Chromatography High Pressure Liquid Pharmacology chemistry.chemical_classification Detection limit Chromatography medicine.diagnostic_test stomatognathic diseases Enzyme Anticonvulsant chemistry Evaluation Studies as Topic Immunoenzyme techniques Immunoassay Phenobarbital Spectrophotometry Ultraviolet medicine.drug |
| Zdroj: | Therapeutic drug monitoring. 15(4) |
| ISSN: | 0163-4356 |
| Popis: | We report the evaluation of the cloned enzyme donor immunoassay (CEDIA) for the estimation of phenytoin and phenobarbital in serum. The assays were performed with a Hitachi 911 analyzer. Intra-assay coefficients of variation were from 1.5 to 4.4% for phenytoin and 1.6 to 5.5% for phenobarbital. Interassay coefficients of variation ranged from 1.8 to 5.3% for phenytoin and 2.9 to 4.8% for phenobarbital. Linearity was satisfactory, with a recovery of 103% at 11 mg/L and 110% at 22 mg/L phenytoin and 93% at 14 mg/L and 101% at 40 mg/L for phenobarbital. The detection limit was 1.2 mg/L for phenytoin and 0.6 mg/L for phenobarbital. Results of this assay correlated well with those of a conventional high-performance liquid chromatography (HPLC) method; r = 0.99 for phenytoin (n = 47) and r = 0.98 for phenobarbital (n = 48). The CEDIA was easy to handle and especially suitable for short turn-around time application. |
| Databáze: | OpenAIRE |
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