Characterization of an area of reference for inhalable particulate matter (PM2.5) associated with genetic biomonitoring in children
| Autor: | Vera Maria Ferrão Vargas, Juliana Marzari Rossato, Cristiane Silva da Silva, Jocelita Aparecida Vaz Rocha |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Health Toxicology and Mutagenesis Toxicology Salmonella Biomonitoring Genetics Animals Humans Organic matter Polycyclic Aromatic Hydrocarbons Child Cells Cultured chemistry.chemical_classification Air Pollutants Micronucleus Tests Strain (chemistry) Chemistry Mouth Mucosa Particulates Rats Comet assay Child Preschool Environmental chemistry Micronucleus test Leukocytes Mononuclear Microsome Female Particulate Matter Comet Assay Seasons Micronucleus Brazil Environmental Monitoring Mutagens |
| Zdroj: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 778:44-55 |
| ISSN: | 1383-5718 |
| Popis: | Humans are exposed to health-impairing air pollutants, especially children who are more sensitive to cancer-causing toxins. This study described an area of reference for inhalable particulates (PM2.5) by chemical (polycyclic aromatic hydrocarbons) and mutagenic characterization associated with the genetic biomonitoring of children (aged 5–11 years). The area studied was in a small town in Brazil, used as reference in previous studies. Organic matter of PM2.5 (extracted with dichloromethane) was evaluated for mutagenesis in a Salmonella /microsome (microsuspension) assay, in strains measuring frameshift error (TA98, YG1021 and YG1024) and base pair substitution (TA100) of DNA, in the presence and absence of rat liver metabolization fraction (S9). Exposure was studied analyzing a sample of 45 children using comet assay (peripheral blood lymphocytes) and micronucleus (exfoliated buccal mucosa cells). PM2.5 concentration for the period was 9% (25.89–64.71 μg/m 3 ) events above WHO limit value (25 μg/m 3 ). Mutagenesis responses (revertants/m 3 ) varied from negative (spring) to 8.3 ± 0.69 (autumn) (−S9) and 5.4 ± 0.36 (winter) (+S9), in strain TA98, and for TA100, in spring, from negative to 14.8 ± 4.23 (−S9) and 17.5 ± 2.72 (+S9). YG strain results show mononitroarenes and aromatic amines. Mean biomonitoring values were established for MN, 0.3 ± 0.41 (‰) and for other cell types a variation from 0.6 ± 0.73 (‰), nuclear buds to 57.5 ± 24.92 (‰), karyorrhexis. Comet assay means were 23.1 ± 12.44; 7.3 ± 11.66 and 0.9 ± 2.30 for tail length, intensity and moment, respectively. There was no difference for sex and age for the different parameters. A significant difference in confounding factors was observed for passive smoking and MN induction. PAHs and mutagenesis in the air may be related to local vehicular emissions. These results challenge the definition of areas of reference for air pollution associated with human biomonitoring including the region studied. |
| Databáze: | OpenAIRE |
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