Listeria monocytogenes infection of HeLa cells results in listeriolysinO-mediated transient activation of the Raf-MEK-MAP kinase pathway
| Autor: | Ulf R. Rapp, Andreas Demuth, Michael Kuhn, Inge Weiglein, Jakob Troppmair, Werner Goebel |
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| Rok vydání: | 2006 |
| Předmět: |
Bacterial Toxins
MAP Kinase Kinase 1 Protein Serine-Threonine Kinases Mitogen-activated protein kinase kinase Microbiology MAP2K7 Hemolysin Proteins Proto-Oncogene Proteins Genetics Humans ASK1 c-Raf Phosphorylation Molecular Biology Heat-Shock Proteins MAPK14 Mitogen-Activated Protein Kinase Kinases MAP kinase kinase kinase Chemistry Protein-Tyrosine Kinases Listeria monocytogenes Molecular biology Cell biology Enzyme Activation Proto-Oncogene Proteins c-raf Calcium-Calmodulin-Dependent Protein Kinases MAP kinase phosphatase Cyclin-dependent kinase 9 HeLa Cells |
| Zdroj: | FEMS Microbiology Letters. 148:189-195 |
| ISSN: | 0378-1097 |
| DOI: | 10.1111/j.1574-6968.1997.tb10287.x |
| Popis: | In this study we investigated the effect of Listeria monocytogenes infection on the activation of the Raf-MEK-MAP kinase pathway in eukaryotic host cells. HeLa cell infection with L. monocytogenes EGD resulted in a rapid, but transient, phosphorylation of the MAP kinases erk-1 and erk-2, a transient phosphorylation of the MAP kinase kinase MEK-1, and a transient activation of the MAP kinase kinase kinase Raf. In parallel to the transient phosphorylation of the MAP kinases, we detected induced expression of the MAP kinase phosphatase MKP-1. Additionally we present evidence that listeriolysin O is the inducing agent for activation of the Raf-MEK-MAP kinase pathway. |
| Databáze: | OpenAIRE |
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