Dynamics of Blood Viral Load Is Strongly Associated with Clinical Outcomes in Coronavirus Disease 2019 (COVID-19) Patients
| Autor: | Xiaolu Long, Hongyan Hou, Ziyong Sun, Liting Chen, Yang Cao, Wei Wang, Gaoxiang Wang, Jiaqi Tan, Lifang Huang, Jianping Zhao, Jianfeng Zhou, Liang Huang, Fankai Meng, Jia Wei, Gang Huang, Na Wang, Weiyong Liu |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty business.industry Viremia medicine.disease medicine.disease_cause Confidence interval Pathology and Forensic Medicine 03 medical and health sciences 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Internal medicine Severity of illness Cohort medicine Molecular Medicine Clinical significance Prospective cohort study business Viral load Coronavirus |
| Zdroj: | The Journal of Molecular Diagnostics |
| ISSN: | 1525-1578 |
| Popis: | The prevalence and clinical relevance of viremia in patients with coronavirus disease 2019 (COVID-19) have not been well studied. A prospective cohort study was designed to investigate blood viral load and clearance kinetics in 52 patients (median age, 62 years; 31 [59.6%] male) and explore their association with clinical features and outcomes based on a novel one-step RT droplet digital PCR (RT-ddPCR). By using one-step RT-ddPCR, 92.3% (48 of 52) of this cohort was quantitatively detected with viremia. The concordance between the blood and oropharyngeal swab tests was 60.92% (53 of 87). One-step RT-ddPCR was tested with a 3.03% false-positive rate and lower 50% confidence interval of detection at 54.026 copies/mL plasma. There was no reduction in the blood viral load in all critical patients, whereas the general and severe patients exhibited a similar ability to clear the viral load. The viral loads in critical patients were significantly higher than those in their general and severe counterparts. Among the 52 study patients, 30 (58%) were discharged from the hospital. Among half of the 30 discharged patients, blood viral load remained positive, of which 76.9% (10 of 13) completely cleared their blood viral load at follow-up. Meanwhile, none of their close contacts had evidence of infection. Quantitative determination of the blood viral test is of great clinical significance in the management of patients with coronavirus disease 2019. |
| Databáze: | OpenAIRE |
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