Sucrose Production Mediated by Lipid Metabolism Suppresses the Physical Interaction of Peroxisomes and Oil Bodies during Germination of Arabidopsis thaliana
| Autor: | Mikio Nishimura, Masayoshi Otomo, Shoji Mano, Makoto Hayashi, Kazusato Oikawa, Songkui Cui, Yasuko Hayashi |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sucrose Mutant Arabidopsis Plant Biology Germination Biology 01 natural sciences Biochemistry 03 medical and health sciences Oil body Organelle Peroxisomes Molecular Biology Adenosine Triphosphatases Arabidopsis Proteins Wild type Lipid metabolism Lipid Droplets Cell Biology Peroxisome Lipid Metabolism biology.organism_classification 030104 developmental biology ATP-Binding Cassette Transporters Energy source Carboxylic Ester Hydrolases 010606 plant biology & botany |
| Zdroj: | Journal of Biological Chemistry. 291:19734-19745 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m116.748814 |
| Popis: | Physical interaction between organelles is a flexible event and essential for cells to adapt rapidly to environmental stimuli. Germinating plants utilize oil bodies and peroxisomes to mobilize storage lipids for the generation of sucrose as the main energy source. Although membrane interaction between oil bodies and peroxisomes has been widely observed, its underlying molecular mechanism is largely unknown. Here we present genetic evidence for control of the physical interaction between oil bodies and peroxisomes. We identified alleles of the sdp1 mutant altered in oil body morphology. This mutant accumulates bigger and more oil body aggregates compared with the wild type and showed defects in lipid mobilization during germination. SUGAR DEPENDENT 1 (SDP1) encodes major triacylglycerol lipase in Arabidopsis. Interestingly, sdp1 seedlings show enhanced physical interaction between oil bodies and peroxisomes compared with the wild type, whereas exogenous sucrose supplementation greatly suppresses the interaction. The same phenomenon occurs in the peroxisomal defective 1 (ped1) mutant, defective in lipid mobilization because of impaired peroxisomal β-oxidation, indicating that sucrose production is a key factor for oil body-peroxisomal dissociation. Peroxisomal dissociation and subsequent release from oil bodies is dependent on actin filaments. We also show that a peroxisomal ATP binding cassette transporter, PED3, is the potential anchor protein to the membranes of these organelles. Our results provide novel components linking lipid metabolism and oil body-peroxisome interaction whereby sucrose may act as a negative signal for the interaction of oil bodies and peroxisomes to fine-tune lipolysis. |
| Databáze: | OpenAIRE |
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