Identification of genetic bases of vibrio fluvialis species-specific biochemical pathways and potential virulence factors by comparative genomic analysis
| Autor: | Biao Kan, Yunduan Wang, Jun Zhu, Xin Lu, Weili Liang, Jialiang Xu |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Virulence Factors Molecular Sequence Data Virulence Genomics Applied Microbiology and Biotechnology Genome Humans Evolutionary and Genomic Microbiology Pathogen Gene Type VI secretion system Vibrio Genetics Ecology biology Genetic Complementation Test Sequence Analysis DNA biology.organism_classification Vibrio fluvialis Genome Bacterial Metabolic Networks and Pathways Food Science Biotechnology |
| Zdroj: | Applied and environmental microbiology. 80(6) |
| ISSN: | 1098-5336 |
| Popis: | Vibrio fluvialis is an important food-borne pathogen that causes diarrheal illness and sometimes extraintestinal infections in humans. In this study, we sequenced the genome of a clinical V. fluvialis strain and determined its phylogenetic relationships with other Vibrio species by comparative genomic analysis. We found that the closest relationship was between V. fluvialis and V. furnissii , followed by those with V. cholerae and V. mimicus . Moreover, based on genome comparisons and gene complementation experiments, we revealed genetic mechanisms of the biochemical tests that differentiate V. fluvialis from closely related species. Importantly, we identified a variety of genes encoding potential virulence factors, including multiple hemolysins, transcriptional regulators, and environmental survival and adaptation apparatuses, and the type VI secretion system, which is indicative of complex regulatory pathways modulating pathogenesis in this organism. The availability of V. fluvialis genome sequences may promote our understanding of pathogenic mechanisms for this emerging pathogen. |
| Databáze: | OpenAIRE |
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