Up-regulation of MARVEL domain-containing protein 1 (MARVELD1) accelerated the malignant phenotype of glioma cancer cells via mediating JAK/STAT signaling pathway
Autor: | Li Binxin, Liye Tan, Tian Wei, Shuang Liang, Jin Peng, Lingyang Xia |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Medicine (General) Physiology Biochemistry Mice 0302 clinical medicine Cell Movement General Pharmacology Toxicology and Pharmaceutics Biology (General) Regulation of gene expression Gene knockdown Brain Neoplasms General Neuroscience JAK-STAT signaling pathway General Medicine Glioma Prognosis Up-Regulation Gene Expression Regulation Neoplastic Phenotype 030220 oncology & carcinogenesis Signal transduction Microtubule-Associated Proteins Signal Transduction Research Article QH301-705.5 Immunology Biophysics Mice Nude Ocean Engineering Biology stat 03 medical and health sciences R5-920 Cell Line Tumor medicine Animals Humans Cell Proliferation MARVELD1 JAK/STAT signaling pathway MARVEL Domain-Containing Proteins Functional analysis Cell growth Membrane Proteins Cell Biology medicine.disease 030104 developmental biology Cancer cell Cancer research |
Zdroj: | Brazilian Journal of Medical and Biological Research, Volume: 54, Issue: 7, Article number: e10236, Published: 17 MAY 2021 Brazilian Journal of Medical and Biological Research v.54 n.7 2021 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Vol 54, Iss 7 (2021) |
Popis: | This work aimed to research the function of MARVEL domain-containing protein 1 (MARVELD1) in glioma as well as its functioning mode. Bioinformatics analysis was utilized to assess the MARVELD1 expression in glioma tissues and its relationship with grade and prognosis, based on The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), and Chinese Glioma Genome Atlas (CGGA) databases. Cell Counting Kit-8 (CCK-8), colony formation, and Transwell assays were carried out to determine the impact of MARVELD1 on malignant biological behavior of glioma, such as proliferation, invasion, and migration. qRT-PCR was carried out to test the mRNA level of MARVELD1. Western blot assay was performed to measure the protein expression of MARVELD1 and JAK/STAT pathway-related proteins. MARVELD1 was expressed at high levels in glioma tissues and cell lines. Kaplan-Meier survival analysis revealed that the higher MARVELD1 expression, the shorter the survival time of patients with glioma. Also, the MARVELD1 expression in WHO IV was significantly enhanced compared to that in WHO II and WHO III. Furthermore, the functional analysis of MARVELD1 in vitro revealed that knockdown of MARVELD1 in U251 cells restrained cell proliferation, migration, and invasion, while up-regulation of MARVELD1 in U87 cells presented opposite outcomes. Finally, we found that JAK/STAT signaling pathway mediated the function of MARVELD1 in glioma. MARVELD1 contributed to promoting the malignant progression of glioma, which is the key driver of activation of JAK/STAT signaling pathway in gliomas. |
Databáze: | OpenAIRE |
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