Up-regulation of MARVEL domain-containing protein 1 (MARVELD1) accelerated the malignant phenotype of glioma cancer cells via mediating JAK/STAT signaling pathway

Autor: Li Binxin, Liye Tan, Tian Wei, Shuang Liang, Jin Peng, Lingyang Xia
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Medicine (General)
Physiology
Biochemistry
Mice
0302 clinical medicine
Cell Movement
General Pharmacology
Toxicology and Pharmaceutics
Biology (General)
Regulation of gene expression
Gene knockdown
Brain Neoplasms
General Neuroscience
JAK-STAT signaling pathway
General Medicine
Glioma
Prognosis
Up-Regulation
Gene Expression Regulation
Neoplastic
Phenotype
030220 oncology & carcinogenesis
Signal transduction
Microtubule-Associated Proteins
Signal Transduction
Research Article
QH301-705.5
Immunology
Biophysics
Mice
Nude
Ocean Engineering
Biology
stat
03 medical and health sciences
R5-920
Cell Line
Tumor
medicine
Animals
Humans
Cell Proliferation
MARVELD1
JAK/STAT signaling pathway
MARVEL Domain-Containing Proteins
Functional analysis
Cell growth
Membrane Proteins
Cell Biology
medicine.disease
030104 developmental biology
Cancer cell
Cancer research
Zdroj: Brazilian Journal of Medical and Biological Research, Volume: 54, Issue: 7, Article number: e10236, Published: 17 MAY 2021
Brazilian Journal of Medical and Biological Research v.54 n.7 2021
Brazilian Journal of Medical and Biological Research
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Brazilian Journal of Medical and Biological Research, Vol 54, Iss 7 (2021)
Popis: This work aimed to research the function of MARVEL domain-containing protein 1 (MARVELD1) in glioma as well as its functioning mode. Bioinformatics analysis was utilized to assess the MARVELD1 expression in glioma tissues and its relationship with grade and prognosis, based on The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), and Chinese Glioma Genome Atlas (CGGA) databases. Cell Counting Kit-8 (CCK-8), colony formation, and Transwell assays were carried out to determine the impact of MARVELD1 on malignant biological behavior of glioma, such as proliferation, invasion, and migration. qRT-PCR was carried out to test the mRNA level of MARVELD1. Western blot assay was performed to measure the protein expression of MARVELD1 and JAK/STAT pathway-related proteins. MARVELD1 was expressed at high levels in glioma tissues and cell lines. Kaplan-Meier survival analysis revealed that the higher MARVELD1 expression, the shorter the survival time of patients with glioma. Also, the MARVELD1 expression in WHO IV was significantly enhanced compared to that in WHO II and WHO III. Furthermore, the functional analysis of MARVELD1 in vitro revealed that knockdown of MARVELD1 in U251 cells restrained cell proliferation, migration, and invasion, while up-regulation of MARVELD1 in U87 cells presented opposite outcomes. Finally, we found that JAK/STAT signaling pathway mediated the function of MARVELD1 in glioma. MARVELD1 contributed to promoting the malignant progression of glioma, which is the key driver of activation of JAK/STAT signaling pathway in gliomas.
Databáze: OpenAIRE
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