Neonatal diet alters fecal microbiota and metabolome profiles at different ages in infants fed breast milk or formula
| Autor: | Katelin S Matazel, Ahmed A. Elolimy, Anne K. Bowlin, Sean H. Adams, Kartik Shankar, Lindsay Pack, Lauren R Brink, Kelly E. Mercer, Thomas M. Badger, Brian D. Piccolo, Aline Andres, Laxmi Yeruva, Sree V. Chintapalli |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Pediatric Research Initiative breastfeeding 030106 microbiology Medicine (miscellaneous) Breast milk Medical and Health Sciences Butyric acid Feces 03 medical and health sciences chemistry.chemical_compound Engineering Betaine microbiota Metabolome Animals Humans Food science metabolites Nutrition Bifidobacterium Pediatric Nutrition and Dietetics Milk Human Bacteria Nutrition & Dietetics biology Prevention Lachnospiraceae Infant biology.organism_classification Infant Formula Bottle Feeding Gastrointestinal Microbiome Original Research Communications immune system Milk Breast Feeding Good Health and Well Being 030104 developmental biology chemistry Female formula diets Breast feeding Human |
| Zdroj: | The American journal of clinical nutrition, vol 111, iss 6 Am J Clin Nutr |
| ISSN: | 0002-9165 |
| Popis: | BackgroundNeonatal diet has a large influence on child health and might modulate changes in fecal microbiota and metabolites.ObjectivesThe aim is to investigate fecal microbiota and metabolites at different ages in infants who were breastfed (BF), received dairy-based milk formula (MF), or received soy-based formula (SF).MethodsFecal samples were collected at 3 (n=16, 12, and 14, respectively), 6 (n=20, 19, and 15, respectively), 9 (n=12, 11, and 12, respectively), and 12 mo (n=14, 14, and 15, respectively) for BF, MF, and SF infants. Infants that breastfed until 9 mo and switched to formula were considered as no longer breastfeeding at 12 mo. Microbiota data were obtained using 16S ribosomal RNA sequencing. Untargeted metabolomics was conducted using a Q-Exactive Hybrid Quadrupole-Orbitrap mass spectrometer. The data were analyzed using R (version 3.6.0) within the RStudio (version 1.1.463) platform.ResultsAt 3, 6, and 9 mo of age BF infants had the lowest α-diversity, SF infants had the highest diversity, and MF was intermediate. Bifidobacterium was 2.6- to 5-fold lower in SF relative to BF infants through 1 y of life. An unidentified genus from Ruminococcaceae higher in the SF (2%) than in the MF (0.4%) and BF (0.08%) infants at 3 mo of age was observed. In BF infants higher levels of butyric acid, d-sphingosine, kynurenic acid, indole-3-lactic acid, indole-3-acetic acid, and betaine were observed than in MF and SF infants. At 3 mo Ruminococcaceae was positively correlated to azelaic, gentisic, isocitric, sebacic, and syringic acids. At 6 mo Oscillospira was negatively correlated with 3-hydroxybutyric-acid, hydroxy-hydrocinnamic acid, and betaine whereas Bifidobacterium was negatively associated with 5-hydroxytryptamine. At 12 mo of age, Lachnospiraceae was negatively associated with hydroxyphenyllactic acid.ConclusionsInfant diet has a large impact on the fecal microbiome and metabolome in the first year of life.This study was registered at clinicaltrials.gov as NCT00616395. |
| Databáze: | OpenAIRE |
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