Substantivity of chlorhexidine to human dentin
| Autor: | Marcela Carrilho, Lorenzo Breschi, Ricardo M. Carvalho, Kelli A. Agee, Leo Tjäderhane, Ethan N. Sousa, David H. Pashley, Jose C. Nicolau, Annalisa Mazzoni, Franklin R. Tay |
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| Přispěvatelé: | Carrilho MR, Carvalho RM, Sousa EN, Nicolau J, Breschi L, Mazzoni A, Tjaderhane L, Tay FR, Agee K, Pashley DH, Carrilho, Mr, Carvalho, Rm, Sousa, En, Nicolau, J, Breschi, Lorenzo, Mazzoni, A, Tjäderhane, L, Tay, Fr, Agee, K, Pashley, D. H. |
| Rok vydání: | 2010 |
| Předmět: |
Molar
DESINFETANTES Materials science CHLORHEXIDINE Dentistry Dental bonding Matrix Metalloproteinase Inhibitors Article chemistry.chemical_compound stomatognathic system Dentin medicine Human dentin Humans Protease Inhibitors General Materials Science dentin bonding agents Tooth Demineralization General Dentistry Phosphoric acid Incubation Analysis of Variance business.industry Chlorhexidine Dental Bonding Durapatite medicine.anatomical_structure chemistry Distilled water Mechanics of Materials Anti-Infective Agents Local business Protein Binding medicine.drug Nuclear chemistry |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Objectives. To better comprehend the role of CHX in the preservation of resin-dentin bonds, this study investigated the substantivity of CHX to human dentin. Material and methods. Dentin disks (n = 45) were obtained from the mid-coronal portion of human third molars. One-third of dentin disks were kept mineralized (MD), while the other two-thirds had one of the surfaces partially demineralized with 37% phosphoric acid for 15 s (PDD) or they were totally demineralized with 10% phosphoric acid (TDD). Disks of hydroxyapatite (HA) were also prepared. Specimens were treated with: (1) 10 mu L of distilled water (controls), (2) 10 mu L of 0.2% chlorhexidine diacetate (0.2% CHX) or (3) 10 mu L of 2% chlorhexidine diacetate (2% CHX). Then, they were incubated in 1 mL of PBS (pH 7.4, 37 degrees C). Substantivity was evaluated as a function of the CHX-applied dose after: 0.5 h, 1 h, 3 h, 6 h, 24 h, 168 h (1 week), 672 h (4 weeks) and 1344 h (8 weeks) of incubation. CHX concentration in eluates was spectrophotometrically analyzed at 260 nm. Results. Significant amounts of CHX remained retained in dentin substrates (MD, PPD or TDD), independent on the CHX-applied dose or time of incubation (p < 0.05). High amounts of retained CHX onto HA were observed only for specimens treated with the highest concentration of CHX (2%) (p < 0.05). Conclusion. The outstanding substantivity of CHX to dentin and its reported effect on the inhibition of dentinal proteases may explain why CHX can prolong the durability of resin-dentin bonds. (C) 2010 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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