A real-time TaqMan PCR assay to discriminate between pathotype 1 (D1) and non-pathotype 1 (D1) isolates of Synchytrium endobioticum
| Autor: | Gerard van Leeuwen, Marga P. E. van Gent-Pelzer, P.J.M. Bonants, Theo van der Lee |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Identification
Pathotype 1 (D1) Synchytrium endobioticum Plant Science Horticulture Biology law.invention law Bioint Diagnostics TaqMan PCR TaqMan Bioassay Cultivar Pathogen Bioint Diagnostics Food Safety & Phyt. Research Polymerase chain reaction Genetics Contig fungi food and beverages biology.organism_classification Detection Food Safety & Phyt. Research Genetic marker Potato wart disease Agronomy and Crop Science |
| Zdroj: | European Journal of Plant Pathology, 143(3), 495-506 European Journal of Plant Pathology 143 (2015) 3 |
| ISSN: | 0929-1873 |
| Popis: | Synchytrium endobioticum is a severe pathogen of potato causing wart disease. For this obligate fungus many pathotypes exist, which are pathogenic or non-pathogenic to different cultivars of potato. To determine to which pathotype an isolate belongs, two biological assays are used on a set of different potato cultivars: the Spieckermann and the Glynne-Lemmerzahl test. A differential set of cultivars has been recommended by EPPO (European Plant Protection Organization) for these tests. Drawbacks of these tests are that it can take up to several months to score the interaction and results are difficult to score and also often not conclusive. Therefore, possibilities were investigated to look for molecular markers for pathotype specificity. An extremely low level of diversity was observed in a set of eight isolates using CRoPS™ (Complexity Reduction of Polymorphic Sequences) technology. Only 191 sequence polymorphisms were found in 14,660 contigs representing approximately 195 Kb of sequence for eight isolates. Nine sequence polymorphisms could be related to pathotype specificity. Two of these polymorphisms were transferred into a real-time TaqMan PCR assay to discriminate between pathotype specific groups. Validation of the assays was performed using multiple isolates from different countries for which the bioassay had been performed. |
| Databáze: | OpenAIRE |
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