Isoliquiritigenin Induces Mitochondrial Dysfunction and Apoptosis by Inhibiting mitoNEET in a Reactive Oxygen Species-Dependent Manner in A375 Human Melanoma Cells
Autor: | Ying Chen, Wenjuan Xu, Wenjin Hao, Kejun Wang, Xiaona Liu, Chuanjun Qu, Huanhuan Ren, Dan Wang, Xiaoyu Chen, Zhaohai Pan, Defang Li, Qiusheng Zheng |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Aging
Article Subject Apoptosis Mitochondrion Biochemistry Mitochondrial Proteins chemistry.chemical_compound Chalcones Cell Line Tumor medicine Humans lcsh:QH573-671 Melanoma Cell Proliferation chemistry.chemical_classification Membrane potential Reactive oxygen species lcsh:Cytology Neurodegeneration Cell Biology General Medicine medicine.disease Mitochondria Cell biology chemistry Cell culture Reactive Oxygen Species Bacterial outer membrane Isoliquiritigenin Research Article |
Zdroj: | Oxidative Medicine and Cellular Longevity, Vol 2019 (2019) Oxidative Medicine and Cellular Longevity |
ISSN: | 1942-0994 1942-0900 |
Popis: | The mitochondrial protein mitoNEET is a type of iron-sulfur protein localized to the outer membrane of mitochondria and is involved in a variety of human pathologies including cystic fibrosis, diabetes, muscle atrophy, and neurodegeneration. In the current study, we found that isoliquiritigenin (ISL), one of the components of the root of Glycyrrhiza glabra L., could decrease the expression of mitoNEET in A375 melanoma cells. We also demonstrated that mitoNEET could regulate the content of reactive oxygen species (ROS), by showing that the ISL-mediated increase in the cellular ROS content could be mitigated by the mitoNEET overexpression. We also confirmed the important role of ROS in ISL-treated A375 cells. The increased apoptosis rate and the decreased mitochondrial membrane potential were mitigated by the overexpression of mitoNEET in A375 cells. These findings indicated that ISL could decrease the expression of mitoNEET, which regulated ROS content and subsequently induced mitochondrial dysfunction and apoptosis in A375 cells. Our findings also highlight mitoNEET as a promising mitochondrial target for cancer therapy. |
Databáze: | OpenAIRE |
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