Osteoclast and osteoblast responsive carbonate apatite coatings for biodegradable magnesium alloys

Autor: Takaya Akashi, Hideki Katayama, Naomi Noda, Sachiko Hiromoto, Tomohiko Yamazaki, Sayaka Itoh
Rok vydání: 2020
Předmět:
30 Bio-inspired and biomedical materials
106 Metallic materials
02 engineering and technology
212 Surface and interfaces
010402 general chemistry
01 natural sciences
Corrosion
stomatognathic system
Osteoclast
carbonate apatite
medicine
General Materials Science
Bone formation
Materials of engineering and construction. Mechanics of materials
Bio-Inspired and Biomedical Materials
corrosion
Mg alloys
Chemistry
food and beverages
Osteoblast
211 Scaffold/Tissue engineering/Drug delivery
021001 nanoscience & nanotechnology
306 Thin film/Coatings
0104 chemical sciences
biomedical magnesium alloy
medicine.anatomical_structure
Chemical engineering
Biodegradable magnesium
Carbonate apatite
osteoclast
osteoblast
TA401-492
600 cytocompatibility
0210 nano-technology
TP248.13-248.65
Research Article
bioabsorbable coating
Biotechnology
Zdroj: Science and Technology of Advanced Materials, Vol 21, Iss 1, Pp 346-358 (2020)
Science and Technology of Advanced Materials
article-version (VoR) Version of Record
ISSN: 1878-5514
1468-6996
DOI: 10.1080/14686996.2020.1761237
Popis: Corrosion-control coatings which can enhance bone formation and be completely replaced by bone are attractive for biodegradable Mg alloys. Carbonate apatite (CAp) and hydroxyapatite (HAp) coatings were formed on Mg-4 wt% Y-3 wt% rare earth (WE43) alloy as a corrosion-control and bioabsorbable coating in the coating solution with various concentrations of NaHCO3. The incorporation of carbonate group in apatite structure was examined using X-ray diffraction and Fourier transform infrared spectroscopy. Rat osteoclast precursor and MC3T3-E1 osteoblast cells were cultured on the CAp- and HAp-coated WE43 to examine the osteoclastic resorption and the alkaline phosphatase (ALP) activity, respectively. Mg ions in the used medium were quantified to examine the corrosion-control ability. The NaHCO3 addition in the solution resulted in the formation of B-type CAp in which the phosphate group of apatite structure was substituted with the carbonate group. The osteoclastic resorption was observed only for the CAp coatings as the cracking of the coatings and the corrosion of substrate WE43 strongly localized under osteoclast cell bodies. The CAp and HAp coatings significantly enhanced the ALP activity of osteoblasts. The CAp-coated WE43 specimens showed 1/5 smaller amount of Mg ion release than the uncoated WE43 on the first day of culturing osteoblasts. For the subsequent 22 days, the Mg ion release was reduced to 1/2 by the CAp coatings. In the presence of osteoclasts, the CAp coatings showed slightly lower corrosion protectiveness than the HAp coating. It was demonstrated that the CAp coatings can be a bioabsorbable and corrosion-control coating for biodegradable Mg alloys.
Databáze: OpenAIRE
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