Sequence-Based Typing of flaB Is a More Stable Screening Tool Than Typing of flaA for Monitoring of Campylobacter Populations
| Autor: | Helge Karch, Edda Bartelt, Andrea Ammon, Dag Harmsen, Jan Mosters, Alexander Mellmann, Alexander W. Friedrich, Peter Roggentin |
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| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Genotype Epidemiology Sequence analysis Biology medicine.disease_cause Disease Outbreaks Campylobacter Infections medicine Animals Humans Typing Insertion sequence Genotyping Genetics Campylobacter Sequence Analysis DNA bacterial infections and mycoses Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field biology.protein bacteria Multilocus sequence typing Cattle Flagellin |
| Zdroj: | Journal of Clinical Microbiology. 42:4840-4842 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.42.10.4840-4842.2004 |
| Popis: | Different typing schemes for Campylobacter spp. were evaluated with 70 outbreak and sporadic isolates. The discriminatory indexes were 0.944 (by pulsed-field gel electrophoresis), 0.920 (by genotyping of the flagellin A gene), 0.902 (by genotyping of flaB ), and 0.886 (by multilocus sequence typing). Cross-classification gave 94.77 or 95.82% (PFGE- flaA or PFGE- flaB ) concordance. flaA was overdiscriminatory in three cases, most probably due to intragenomic recombination. |
| Databáze: | OpenAIRE |
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