Resistance exercise attenuates IKKε phosphorylation and hepatic fat accumulation of obese mice

Autor:	Gabriel Calheiros Antunes, Robson Damasceno de Lima, Renan Fudoli Lins Vieira, Ana Paula Azevêdo Macêdo, Vitor Rosetto Muñoz, Erika Pereira Zambalde, Caio Felipe Romeiro, Fernando Moreira Simabuco, Patricia Oliveira Prada, Adelino Sanchez Ramos da Silva, Eduardo Rochete Ropelle, Dennys Esper Cintra, José Rodrigo Pauli
Rok vydání:	2022
Předmět:	Pharmacology Male Physiology Mice Obese Resistance Training Diet High-Fat I-kappa B Kinase Mice Inbred C57BL Mice Glucose Liver Physiology (medical) Animals Humans Obesity Insulin Resistance Phosphorylation Adiposity
Zdroj:	Clinical and experimental pharmacologyphysiologyREFERENCES. 49(10)
ISSN:	1440-1681
Popis:	Obesity is associated with low-grade inflammation and disturbances in hepatic metabolism. This study aimed to investigate the effects of resistance exercise on inflammatory signalling related to IκB kinase (IKK) ɛ protein (IKKɛ) and on hepatic fat accumulation in obese mice. Male Swiss mice were distributed into three groups: control (CTL) fed with standard chow; obese (OB) mice induced by a high-fat diet (HFD); obese exercised (OB + RE) mice fed with HFD and submitted to a resistance exercise training. The resistance exercise training protocol consisted of 20 sets/3 ladder climbs for 8 weeks, three times/week on alternate days. The training overload was equivalent to 70% of the maximum load supported by the rodent. Assays were performed to evaluate weight gain, hepatic fat content, fasting glucose, insulin sensitivity, IKKɛ phosphorylation and proteins related to insulin signalling and lipogenesis in the liver. Mice that received the high-fat diet showed greater adiposity, impaired insulin sensitivity, increased fasting glucose and increased hepatic fat accumulation. These results were accompanied by an increase in IKKɛ phosphorylation and lipogenesis-related proteins such as cluster of differentiation 36 (CD36) and fatty acid synthase (FAS) in the liver of obese mice. In contrast, exercised mice showed lower body weight and adiposity evolution throughout the experiment. In addition, resistance exercise suppressed the effects of the high-fat diet by reducing IKKɛ phosphorylation and hepatic fat content. In conclusion, resistance exercise training improves hepatic fat metabolism and glycaemic homeostasis, which are, at least in part, linked to the anti-inflammatory effect of reduced IKKɛ phosphorylation in the liver of obese mice.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b70848e9fd5048b5dbc5f1ffc7d8b13a https://pubmed.ncbi.nlm.nih.gov/35690890 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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