Physiological evidence of mitochondrial permeability transition pore opening caused by lipid deposition leading to hepatic steatosis in db/db mice
| Autor: | Min Yang, Qinyi Wang, Hui Shi, Yuan Li, Jing Wu, Hong-Kun Wu, Lisi Wei |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Mice Inbred Strains Mitochondrion Mitochondrial Membrane Transport Proteins Biochemistry Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Physiology (medical) medicine Animals Membrane potential Mitochondrial Permeability Transition Pore MPTP Depolarization Lipid Metabolism medicine.disease Lipids Mitochondria Cell biology Fatty Liver Calcein 030104 developmental biology Mitochondrial permeability transition pore chemistry Apoptosis Calcium Steatosis 030217 neurology & neurosurgery |
| Zdroj: | Free Radical Biology and Medicine. 162:523-532 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/j.freeradbiomed.2020.11.009 |
| Popis: | Mitochondrial permeability transition pore (mPTP) is an important regulator in cell apoptosis and necrosis. However, its role in hepatic steatosis, especially its electrophysiological properties transformation remains elusive. Herein, using diabetes mice, we investigated the role of mPTP in hepatic steatosis triggered by diabetes and the mechanisms involved. We found that hepatic steatosis altered mitochondrial morphology, generating mega mitochondria, mitochondria swelling, calcein fluorescence quenching and mitochondrial membrane potential depolarization. At the same time, we confirmed an augmented mPTP opening with patch clamping in liver mitoplasts in db/db mice and a similar transformation with arachidonic acid (AA) simulating liquid deposition. We also found mPTP opening was significantly attenuated in wt mice after removing mitochondrial matrix, while that in db/db mice remained active. In addition, we observed that AA could directly activate mPTP in inside-out mode, independent of matrix calcium. In conclusion, we for the first time provided a physiological evidence of mPTP opening in lipid deposition, which could be directly induced by AA without Ca2+ and can be inhibited by cyclosporine A. As a result, it led to mitochondria morphology and function transformation. This might provide insights into potential therapeutic target for future treatment of mitochondrial liver disease. |
| Databáze: | OpenAIRE |
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