Expression and functional activity of PACAP and its receptors on cumulus cells: Effects on oocyte maturation

Autor: Virginia Di Paolo, Marzia Barberi, Stefania Latini, Sandra Cecconi, Maria Cristina Guglielmo, Rita Canipari
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Male
Cytoplasm
8-Bromo Cyclic Adenosine Monophosphate
Apoptosis
Chorionic Gonadotropin
Biochemistry
Human chorionic gonadotropin
Mice
Endocrinology
Receptor
Cells
Cultured
Fertilisation
media_common
Cumulus Cells
Cell biology
medicine.anatomical_structure
Intercellular Signaling Peptides and Proteins
Pituitary Adenylate Cyclase-Activating Polypeptide
Receptors
Vasoactive Intestinal Peptide
Type II
Female
Gonadotropin
hormones
hormone substitutes
and hormone antagonists
Ovulation
EGF Family of Proteins
endocrine system
medicine.medical_specialty
medicine.drug_class
media_common.quotation_subject
Adenylate kinase
Fertilization in Vitro
Biology
Amphiregulin
Internal medicine
medicine
Animals
Humans
RNA
Messenger
Molecular Biology
Cell Proliferation
Glycoproteins
Cell Nucleus
Epidermal Growth Factor
urogenital system
Oocyte
Oocytes
Sperm Head
Follicle Stimulating Hormone
Receptors
Pituitary Adenylate Cyclase-Activating Polypeptide
Type I
Popis: Pituitary adenylate cyclase-activating polypeptide (PACAP) and its receptor PAC1-R (PACAP type 1 receptor) are transiently expressed in granulosa cells (GCs) of mouse preovulatory follicles and affect several parameters associated with the ovulatory process. We investigated the expression of PACAP and its receptors in cumulus cells (CCs) after the LH surge and their role on cumulus expansion/apoptosis and oocyte maturation. PACAP and PAC1-R expression increased in CCs isolated at different times after treatment with human chorionic gonadotropin (hCG). Moreover, PACAP was able to reverse the inhibition of oocyte meiotic maturation caused by hypoxantine in cumulus cell-oocyte complexes (COCs) and efficiently promoted male pronuclear formation after fertilisation. PACAP was also able to induce cumulus expansion and prevent CC apoptosis. Our results demonstrated the induction of PACAP and its receptors in CCs by LH and EGF, suggesting that PACAP may play a significant role in the complex interactions of gonadotropin and growth factors during ovulation and fertilisation.
Databáze: OpenAIRE
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