| Autor: |
Kuldeep Singh Chauhan, Annesa Das, Hemant Jaiswal, Irene Saha, Monika Kaushik, Virendra Kumar Patel, Prafullakumar Tailor |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Cellular Immunology. 371:104468 |
| ISSN: |
0008-8749 |
| DOI: |
10.1016/j.cellimm.2021.104468 |
| Popis: |
Dendritic cells (DCs) play central role in innate as well as adaptive immune responses regulated by diverse DC subtypes that vary in terms of surface markers, transcriptional profile and functional responses. Generation of DC diversity from progenitor stage is tightly regulated by complex molecular inter-play between transcription factors. We earlier demonstrated that Batf3 and Id2 expression have a synergistic effect on the Irf8 directed classical cDC1 development. In present study, Bi-molecular fluorescence complementation assay suggested that IRF8 interacts with BATF3, and ID2 may aid cDC1 development independently. Genome wide recruitment analysis of IRF8 and BATF3 from different DC subtypes led to identification of the overlapping regions of occupancy by these two transcription factors. Further analysis of overlapping peaks of IRF8 and BATF3 occupancy in promoter region within the cDC1 subtype specific transcriptional pattern identified a metabolically important Pfkfb3 gene. Among various immune cell types; splenic cDC1 subtype displayed enhanced expression of Pfkfb3. Analysis of Irf8 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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